Kuppusamy Saranya, Thavamani Palanisami, Megharaj Mallavarapu, Lee Yong Bok, Naidu Ravi
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, South Korea; Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA5095, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia.
Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA5106, Australia; Global Centre for Environmental Remediation (GCER), Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
Chemosphere. 2016 Nov;162:31-9. doi: 10.1016/j.chemosphere.2016.07.052. Epub 2016 Jul 28.
An isolate of Cupriavidus (strain MTS-7) was identified from a long-term PAHs and heavy metals mixed contaminated soil with the potential to biodegrade both LMW and HMW PAHs with added unique traits of acid and alkali tolerance, heavy metal tolerance, self-nutrient assimilation by N fixation and P solubilization. This strain completely degraded the model 3 (150 mg L(-1) Phe), 4 (150 mg L(-1) Pyr) and 5 (50 mg L(-1) BaP) ring PAHs in 4, 20 and 30 days, respectively. It could mineralize 90-100% of PAHs (200 mg L(-1) of Phe and Pyr) within 15 days across pH ranging from 5 to 8 and even in the presence of toxic metal contaminations. During biodegradation, the minimum inhibitory concentrations were 5 (Cu(2+)) and 3 (Cd(2+), Pb(2+), Zn(2+)) mg L(-1) of the potentially bioavailable metal ions and over 17 mg L(-1) metal levels was lethal for the microbe. Further, it could fix 217-274 μg mL(-1) of N and solubilize 79-135 μg mL(-1) of P while PAHs degradation. MTS-7 as a superior candidate could be thus used in the enhanced bioaugmentation and/or phytoremediation of long-term mixed contaminated sites.
从长期受多环芳烃和重金属混合污染的土壤中分离出一株贪铜菌（菌株MTS-7），该菌株具有同时生物降解低分子量和高分子量多环芳烃的潜力，并具有耐酸耐碱、耐重金属、通过固氮和溶磷实现自营养同化等独特特性。该菌株分别在4天、20天和30天内完全降解了模型3（150 mg L⁻¹ 菲）、4（150 mg L⁻¹ 芘）和5（50 mg L⁻¹ 苯并[a]芘）环多环芳烃。在pH值为5至8的范围内，甚至在存在有毒金属污染的情况下，它能在15天内将90 - 100%的多环芳烃（200 mg L⁻¹ 的菲和芘）矿化。在生物降解过程中，潜在生物可利用金属离子的最低抑制浓度分别为5（Cu²⁺）和3（Cd²⁺、Pb²⁺、Zn²⁺）mg L⁻¹，超过17 mg L⁻¹ 的金属水平对微生物具有致死性。此外，在多环芳烃降解过程中，它能固定217 - 274 μg mL⁻¹ 的氮并溶解79 - 135 μg mL⁻¹ 的磷。因此，MTS-7作为一种优良候选菌株可用于长期混合污染场地的强化生物强化和/或植物修复。
