Jin Lei, Garamus Vasil M, Liu Fang, Xiao Jingwen, Eckerlebe Helmut, Willumeit-Römer Regine, Mu Bozhong, Zou Aihua
Shanghai Key Laboratory of Functional Materials Chemistry and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Institute of Materials Research, Max-Planck-Strasse 1, 21502 Geesthacht, Germany.
J Colloid Interface Sci. 2016 Nov 1;481:201-9. doi: 10.1016/j.jcis.2016.07.044. Epub 2016 Jul 19.
The interaction between biosurfactant Surfactin and cationic Gemini surfactant ethanediyl-1,3-bis(dodecyldimethylammonium bromide) (abbreviated as 12-3-12) was investigated using turbidity, surface tension, dynamic light scattering (DLS) and small angle neutron scattering (SANS). Analysis of critical micelle concentration (CMC) values in Surfactin/12-3-12 mixture indicates that there is synergism in formation of mixed Surfactin/12-3-12 micelles. Although Surfactin and 12-3-12 are oppositely charged in phosphate buffer solution (PBS, pH7.4), there are no precipitates observed at the concentrations below the CMC of Surfactin/12-3-12 system. However, at the concentration above CMC value, the Surfactin/12-3-12 mixture is severely turbid with high 12-3-12 content. DLS and SANS measurements follow the size and shape changes of mixed Surfactin/12-3-12 aggregates from small spherical micelles via elongated aggregates to large bulk complexes with increasing fraction of Gemini surfactant.
使用浊度、表面张力、动态光散射（DLS）和小角中子散射（SANS）研究了生物表面活性剂表面活性素与阳离子双子表面活性剂1,3 - 二（十二烷基二甲基溴化铵）乙二酯（简称为12 - 3 - 12）之间的相互作用。对表面活性素/12 - 3 - 12混合物中临界胶束浓度（CMC）值的分析表明，在形成混合的表面活性素/12 - 3 - 12胶束方面存在协同作用。尽管在磷酸盐缓冲溶液（PBS，pH7.4）中表面活性素和12 - 3 - 12带相反电荷，但在低于表面活性素/12 - 3 - 12体系CMC的浓度下未观察到沉淀。然而，在高于CMC值的浓度下，表面活性素/12 - 3 - 12混合物在12 - 3 - 12含量高时会严重浑浊。随着双子表面活性剂比例的增加，DLS和SANS测量跟踪了混合的表面活性素/12 - 3 - 12聚集体从小球形胶束通过伸长的聚集体到大型块状复合物的尺寸和形状变化。
