Kim Shin Hye, Lee Su Hyeon, Lee Won-Yong, Lee Jeongae, Chung Bong Chul, Park Mi-Jung, Choi Man Ho
Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul 01757, Republic of Korea.
Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
Clin Chim Acta. 2016 Oct 1;461:47-52. doi: 10.1016/j.cca.2016.07.022. Epub 2016 Jul 27.
The study aim was to evaluate the correlation of sexual dimorphism and pubertal stage with steroid metabolism in adolescents.
The serum levels of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) and their sulfate conjugates were quantitatively profiled from serum samples of 199 adolescents (87 boys and 112 girls), aged 6 to 14years.
In the prepubertal stage, DHEA levels in girls were higher than those in boys; however, significantly increased DHEA levels during pubertal development in boys. Pregnenolone levels were significantly higher in girls; however, the levels of its sulfate were higher in boys. The serum levels of both cholesterol and its sulfate were higher in boys, especially in the early to mid-pubertal stages. DHEA and DHEA sulfate levels in both sexes significantly increased with pubertal development (P for trend<0.05), while pregnenolone, cholesterol, and cholesterol sulfate in both sexes were stable. The metabolic ratios, indicating sulfotransferase activity, were significantly higher in boys, and increased with pubertal development in boys, but not in girls, while CYP11A1 activity levels increased significantly in both sexes with pubertal development.
Sexual dimorphism in key enzymes of androgen biosynthesis during pubertal changes may help elucidate the normal physiology of steroidogenesis.
本研究旨在评估青少年性二态性和青春期阶段与类固醇代谢的相关性。
对199名6至14岁青少年（87名男孩和112名女孩）的血清样本进行定量分析，检测胆固醇、孕烯醇酮、脱氢表雄酮（DHEA）及其硫酸酯结合物的血清水平。
青春期前阶段，女孩的DHEA水平高于男孩；然而，男孩在青春期发育期间DHEA水平显著升高。女孩的孕烯醇酮水平显著更高；然而，其硫酸酯水平在男孩中更高。男孩的胆固醇及其硫酸酯的血清水平均更高，尤其是在青春期早期至中期。随着青春期发育，男女两性的DHEA和硫酸脱氢表雄酮水平均显著升高（趋势P<0.05），而男女两性的孕烯醇酮、胆固醇和胆固醇硫酸酯水平保持稳定。表明硫酸转移酶活性的代谢比率在男孩中显著更高，且随男孩青春期发育而增加，但在女孩中并非如此，而CYP11A1活性水平在男女两性中均随青春期发育而显著增加。
青春期变化过程中雄激素生物合成关键酶的性二态性可能有助于阐明类固醇生成的正常生理机制。
