Pulver Ross J, Campbell Phillip M, Opperman Lynne A, Buschang Peter H
Private practice, Houston, Tex.
Chairman, Department of Orthodontics, Texas A&M University Baylor College of Dentistry, Dallas, Tex.
Am J Orthod Dentofacial Orthop. 2016 Aug;150(2):303-12. doi: 10.1016/j.ajodo.2015.12.026.
In this study, we experimentally evaluated whether complex, mature sutures can be separated using skeletal anchorage and light, continuous forces.
Twelve adult, 8- to 9-month-old female New Zealand white rabbits were randomly assigned to 1 control group and 2 experimental groups. Open-coil nickel-titanium springs delivered constant forces of 100 g across the sagittal suture to miniscrew implants placed bilaterally in the frontal bone. Sutural separation was measured biweekly. Separation was also measured with microcomputed tomography. Bone formation (mineral apposition) was measured with fluorescent labels. Qualitative histologic analyses of the suture tissues were performed using hematoxylin and eosin staining; osteoclasts were evaluated with tartrate resistant acid phosphatase staining.
All 24 miniscrew implants remained stable throughout the experiment. There was no statistically significant sutural separation in the control group. In the experimental groups, sutural separation was significant (P <0.05) at all time points after the initial records were taken. The rate of separation was linear during the first 42 days. There were moderate correlations (R = 0.59-0.89; P <0.05) between miniscrew implant separation and bone marker separation. Mineral apposition rate, which was not measureable in the control group, was significant in the experimental group. The mineral apposition rate was greater between 14 and 28 days than between 28 and 38 days, and it was greater on the ectocranial than on the endocranial surface. Based on the microcomputed tomography analysis, 3-dimensional sutural volume of the experimental group increased significantly (P = 0.02), but surface area did not (P = 0.26).
It is possible to separate the sagittal suture of mature rabbits. Sutural separation is limited, indicating involvement of other articulations.
在本研究中，我们通过实验评估了能否使用骨骼锚固和轻柔、持续的力来分离复杂、成熟的缝线。
将12只8至9个月大的成年雌性新西兰白兔随机分为1个对照组和2个实验组。开放式镍钛弹簧通过矢状缝向双侧置于额骨的微型螺钉植入物施加100克的恒定力。每两周测量一次缝线分离情况。还通过微型计算机断层扫描测量分离情况。用荧光标记物测量骨形成（矿物质沉积）。使用苏木精和伊红染色对缝线组织进行定性组织学分析；用抗酒石酸酸性磷酸酶染色评估破骨细胞。
在整个实验过程中，所有24个微型螺钉植入物均保持稳定。对照组中没有统计学上显著的缝线分离。在实验组中，在首次记录后的所有时间点，缝线分离均显著（P<0.05）。在最初的42天内，分离速率呈线性。微型螺钉植入物分离与骨标志物分离之间存在中度相关性（R = 0.59 - 0.89；P<0.05）。对照组中无法测量的矿物质沉积率在实验组中显著。矿物质沉积率在14至28天之间高于28至38天之间，且在颅外表面大于颅内表面。基于微型计算机断层扫描分析，实验组的三维缝线体积显著增加（P = 0.02），但表面积未增加（P = 0.26）。
分离成熟兔的矢状缝是可能的。缝线分离是有限的，表明其他关节也参与其中。
