Zimmermann Gunther, Neri Dario
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH Zürich), Vladimir Prelog Weg 1-5/10, CH-8093 Zürich, Switzerland.
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH Zürich), Vladimir Prelog Weg 1-5/10, CH-8093 Zürich, Switzerland.
Drug Discov Today. 2016 Nov;21(11):1828-1834. doi: 10.1016/j.drudis.2016.07.013. Epub 2016 Jul 28.
DNA-encoded chemical libraries have emerged as a powerful tool for hit identification in the pharmaceutical industry and in academia. Similar to biological display techniques (such as phage display technology), DNA-encoded chemical libraries contain a link between the displayed chemical building block and an amplifiable genetic barcode on DNA. Using routine procedures, libraries containing millions to billions of compounds can be easily produced within a few weeks. The resulting compound libraries are screened in a single test tube against proteins of pharmaceutical interest and hits can be identified by PCR amplification of DNA barcodes and subsequent high-throughput sequencing.
DNA编码化学文库已成为制药行业和学术界用于发现活性分子的强大工具。与生物展示技术（如噬菌体展示技术）类似，DNA编码化学文库在展示的化学结构单元与DNA上可扩增的遗传条形码之间存在联系。通过常规程序，可在几周内轻松制备包含数百万至数十亿种化合物的文库。所得化合物文库在单个试管中针对具有药学意义的蛋白质进行筛选，通过对DNA条形码进行PCR扩增及随后的高通量测序来鉴定活性分子。
