Szczeszak Agata, Ekner-Grzyb Anna, Runowski Marcin, Szutkowski Kosma, Mrówczyńska Lucyna, Kaźmierczak Zuzanna, Grzyb Tomasz, Dąbrowska Krystyna, Giersig Michael, Lis Stefan
Adam Mickiewicz University, Faculty of Chemistry, Department of Rare Earths, Umultowska 89b, 61-614 Poznań, Poland.
Adam Mickiewicz University, Faculty of Biology, Umultowska 89, 61-614 Poznań, Poland.
J Colloid Interface Sci. 2016 Nov 1;481:245-55. doi: 10.1016/j.jcis.2016.07.025. Epub 2016 Jul 15.
The luminescent GdVO4:Eu(3+)5%@SiO2@NH2 core@shell nanomaterials were obtained via co-precipitation method, followed by hydrolysis and co-condensation of silane derivatives: tetraethyl orthosilicate and 3-aminopropyltriethoxysilane. Their effect on human erythrocytes sedimentation and on proliferation of human lung microvascular endothelial cells was examined and discussed. The luminescent nanoparticles were synthesized in the presence of polyacrylic acid or glycerin in order to minimalize the agglomeration and excessive growth of nanostructures. Surface coating with amine functionalized silica shell improved their biocompatibility, facilitated further organic conjugation and protected the internal core. Magnetic measurements revealed an enhanced T1-relaxivity for the synthesized GdVO4:Eu(3+)5% nanostructures. Structure, morphology and average grain size of the obtained nanomaterials were determined by X-ray diffraction, transmission electron microscopy and dynamic light scattering analysis. The qualitative elemental composition of the nanomaterials was established using energy-dispersive X-ray spectroscopy. The spectroscopic characteristic of red emitting core@shell nanophosphors was completed by measuring luminescence spectra and decays. The emission spectra revealed characteristic bands of Eu(3+) ions related to the transitions (5)D0-(7)F0,1,2,3,4 and (5)D1-(7)F1. The luminescence lifetimes consisted of two components, associated with the presence of Eu(3+) ions located at the surface of the crystallites and in the bulk.
通过共沉淀法，随后进行硅烷衍生物（正硅酸四乙酯和3-氨丙基三乙氧基硅烷）的水解和共缩合反应，制备了发光的GdVO4:Eu(3+)5%@SiO2@NH2核壳纳米材料。研究并讨论了它们对人红细胞沉降和人肺微血管内皮细胞增殖的影响。发光纳米颗粒是在聚丙烯酸或甘油存在的情况下合成的，以尽量减少纳米结构的团聚和过度生长。用胺官能化二氧化硅壳进行表面包覆提高了它们的生物相容性，便于进一步的有机缀合，并保护内部核心。磁性测量表明，合成的GdVO4:Eu(3+)5%纳米结构的T1弛豫率有所提高。通过X射线衍射、透射电子显微镜和动态光散射分析确定了所得纳米材料的结构、形态和平均晶粒尺寸。使用能量色散X射线光谱法确定了纳米材料的定性元素组成。通过测量发光光谱和衰减完成了红色发射核壳纳米磷光体的光谱特性。发射光谱揭示了与跃迁(5)D0-(7)F0,1,2,3,4和(5)D1-(7)F1相关的Eu(3+)离子的特征带。发光寿命由两个成分组成，与位于微晶表面和体相中的Eu(3+)离子的存在有关。
