Grandier-Vazeille X, Duvert M, Chevallier J
Laboratoire de Cytologie, UA-CNRS 339, Talence, France.
Neurosci Lett. 1989 Apr 24;99(1-2):30-4. doi: 10.1016/0304-3940(89)90259-0.
Polyclonal auto-anti-idiotypic antibodies (AAIs), detected in antisera of rabbits immunized with acetylcholine conjugate, have already been characterized. In this paper, we report on a cytochemical application of these AAIs on skeletal muscle motor endplates of an invertebrate. We have shown that there is a non-uniformly distributed reaction in the synaptic cleft. The deposits seem to be associated with the muscular plasma membrane. The specificity of the cytochemical method is discussed. On this kind of muscle, the AAIs are able to detect, at the ultrastructural level, acetylcholine binding sites which most likely are acetylcholine receptors.
在以乙酰胆碱结合物免疫的兔抗血清中检测到的多克隆自身抗独特型抗体（AAIs）已得到表征。在本文中，我们报道了这些AAIs在一种无脊椎动物骨骼肌运动终板上的细胞化学应用。我们已经表明，在突触间隙存在分布不均匀的反应。沉积物似乎与肌细胞膜相关。讨论了细胞化学方法的特异性。在这种肌肉上，AAIs能够在超微结构水平检测到最有可能是乙酰胆碱受体的乙酰胆碱结合位点。
