乌干达的乙型肝炎病毒基因型 A 和 D。
Hepatitis B virus genotypes A and D in Uganda.
机构信息
Department of Medicine , Makerere University College of Health Sciences, Mulago Hospital , Kampala , Uganda.
Department of Microbiology , Makerere University College of Health Sciences , Kampala , Uganda.
出版信息
J Virus Erad. 2016 Jan 1;2(1):19-21. doi: 10.1016/S2055-6640(20)30693-2.
BACKGROUND
The prevalence of hepatitis B virus (HBV) infection in Uganda is 10%. Hepatitis B virus genotypes impact on treatment response, rate of spontaneous recovery and progression of chronic HBV infection and hepatocellular carcinoma. There is little information on the HBV genotypic distribution in Uganda.
OBJECTIVES
To determine HBV genotypes in Uganda.
METHODS
The MBN clinical laboratory performs HBV viral load and genotype testing in Uganda. It receives hepatitis B surface antigen (HBsAg)-positive samples from all over the country for additional HBV testing. Samples are stored for 6 months before being discarded. Our study used delinked stored samples. PCR-positive samples had DNA extracted and used as template for HBV genome amplification by nested PCR. Reverse hybridisation was performed and genotypes were determined by the line probe assay method (INNO-LiPA).
RESULTS
One hundred stored HBsAg-positive plasma samples with detectable viral loads were analysed. Of these, 93 samples showed PCR amplification products and gave genotype-specific probe lines on the INNO-LiPA assay. Of the patients, where gender was recorded, 60.9% were female, and the overall median age (IQR) was 25 (2-60) years. There was a predominance of HBV genotype D (47 patients; 50.5%), followed by genotype A, (16 patients; 17.2%). One patient (1.1%) had genotype E. In 28% of the samples mixed infections were detected with genotypes A/E (9.7%) and A/D (6.5%) being most common. Genotypes B, C, E and H only occurred as part of mixed infections.
CONCLUSION
Hepatitis B genotypes D and A were predominant in our study population.
背景
在乌干达，乙型肝炎病毒（HBV）感染的流行率为 10%。HBV 基因型影响治疗反应、自发恢复率以及慢性 HBV 感染和肝细胞癌的进展。关于乌干达 HBV 基因型分布的信息较少。
目的
确定乌干达的 HBV 基因型。
方法
MBN 临床实验室在乌干达进行 HBV 病毒载量和基因型检测。它从全国各地接收乙型肝炎表面抗原（HBsAg）阳性样本，进行额外的 HBV 检测。样本在丢弃前储存 6 个月。我们的研究使用了脱链储存的样本。PCR 阳性样本提取 DNA，用作巢式 PCR 扩增 HBV 基因组的模板。进行反向杂交，通过线探针分析（INNO-LiPA）方法确定基因型。
结果
分析了 100 份储存的 HBsAg 阳性、可检测病毒载量的血浆样本。其中，93 份样本显示 PCR 扩增产物，并在 INNO-LiPA 检测中出现基因型特异性探针线。在记录了性别信息的患者中，60.9%为女性，总体中位数（IQR）年龄为 25（2-60）岁。HBV 基因型 D 占优势（47 例；50.5%），其次是基因型 A（16 例；17.2%）。1 例（1.1%）患者为基因型 E。在 28%的样本中检测到混合感染，其中 A/E（9.7%）和 A/D（6.5%）最为常见。基因型 B、C、E 和 H 仅作为混合感染的一部分发生。
结论
在我们的研究人群中，乙型肝炎基因型 D 和 A 占主导地位。
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