坦桑尼亚西北部乙肝病毒基因多样性的模式变化

The pattern change of hepatitis B virus genetic diversity in Northwestern Tanzania.

作者信息

Mlewa Mathias, Henerico Shimba, Nyawale Helmut A, Mangowi Ivon, Shangali Aminiel Robert, Manisha Anselmo Mathias, Kisanga Felix, Kidenya Benson R, Jaka Hyasinta, Kilonzo Semvua B, Groendahl Britta, Koliopoulos Philipp, Stephan Gehring, Ntinginya Nyanda Elias, Mirambo Mariam M, Mshana Stephen E

机构信息

Department of Microbiology and Immunology, Mwanza University, Kishiri, P.O. Box 3068, Mwanza, Tanzania.

Department of Microbiology and Immunology, Catholic University of Health, and Allied Sciences, Bugando, P.O. Box 1464, Mwanza, Tanzania.

出版信息

Sci Rep. 2025 Mar 7;15(1):8021. doi: 10.1038/s41598-025-89303-7.

DOI:10.1038/s41598-025-89303-7

PMID:40055372

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11889104/

Abstract

Hepatitis B virus genetic diversity (HBV) evaluation is scarcely done in Tanzania, imposing a crucial knowledge gap toward elimination of HBV infection by 2030. This cross-sectional study was conducted on purposively selected 21 plasma samples with high HBV-deoxyribonucleic acid (DNA) levels of > 300,000IU/mL. DNA extraction was done using Qiagen DNA Blood Mini Kit (Qiagen, Hilden, Germany). Partial amplification of 423 bp of pol gene, sequencing and analysis; and statistical analysis by STATA version 15 were done. These patients had mean age of 41 ± 11 years with HBV-DNA median of 979 [185.5-8457.5] IU/mL. The genotypes detected were HBV/A; 76.2% (16/21), HBV/D; 19% (4/21), and lastly HBV/G; 4.8% (1/21). Most of the HBV/As and all of the HBV/Ds identified in this study did not cluster with HBV/As and HBV/Ds from other parts of the world. Overall, 19% (4/21) of the patients had HBV escape mutations (T123V, Y134N, P120T and T123A). In conclusion, HBV/A and HBV/D are predominant over time in North-western Tanzania. Most HBV/A and all HBV/D are unique to Tanzania as had been previously reported. However, the pattern of hepatitis B virus genetic diversity is changing in Northwestern Tanzania with occurrence of HBV/G as new genotype in the region.

摘要

在坦桑尼亚，很少进行乙型肝炎病毒（HBV）基因多样性评估，这给到2030年消除HBV感染造成了关键的知识空白。本横断面研究针对有目的地选取的21份HBV脱氧核糖核酸（DNA）水平>300,000IU/mL的血浆样本开展。使用Qiagen DNA血液微型试剂盒（Qiagen，德国希尔德）进行DNA提取。对pol基因的423bp进行部分扩增、测序和分析；并使用STATA 15版本进行统计分析。这些患者的平均年龄为41±11岁，HBV-DNA中位数为979[185.5-8457.5]IU/mL。检测到的基因型为HBV/A；76.2%（16/21），HBV/D；19%（4/21），最后是HBV/G；4.8%（1/21）。本研究中鉴定出的大多数HBV/A和所有HBV/D均未与来自世界其他地区的HBV/A和HBV/D聚类。总体而言，19%（4/21）的患者存在HBV逃逸突变（T123V、Y134N、P120T和T123A）。总之，随着时间推移，HBV/A和HBV/D在坦桑尼亚西北部占主导地位。如先前报道，大多数HBV/A和所有HBV/D在坦桑尼亚是独特的。然而，坦桑尼亚西北部的乙型肝炎病毒基因多样性模式正在发生变化，HBV/G作为该地区的新基因型出现。