利用吸附法进行原位产物回收的丙酮-丁醇-乙醇发酵生产丁醇。
Butanol production in acetone-butanol-ethanol fermentation with in situ product recovery by adsorption.
机构信息
Department of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
出版信息
Bioresour Technol. 2016 Nov;219:158-168. doi: 10.1016/j.biortech.2016.07.111. Epub 2016 Jul 27.
Activated carbon Norit ROW 0.8, zeolite CBV901, and polymeric resins Dowex Optipore L-493 and SD-2 with high specific loadings and partition coefficients were studied for n-butanol adsorption. Adsorption isotherms were found to follow Langmuir model, which can be used to estimate the amount of butanol adsorbed in acetone-butanol-ethanol (ABE) fermentation. In serum-bottle fermentation with in situ adsorption, activated carbon showed the best performance with 21.9g/L of butanol production. When operated in a fermentor, free- and immobilized-cell fermentations with adsorption produced 31.6g/L and 54.6g/L butanol with productivities of 0.30g/L·h and 0.45g/L·h, respectively. Thermal desorption produced a condensate containing ∼167g/L butanol, which resulted in a highly concentrated butanol solution of ∼640g/L after spontaneous phase separation. This in situ product recovery process with activated carbon is energy efficient and can be easily integrated with ABE fermentation for n-butanol production.
研究了具有高比载和分配系数的活性炭 Norit ROW 0.8、沸石 CBV901 以及聚合物树脂 Dowex Optipore L-493 和 SD-2 对正丁醇的吸附。发现吸附等温线符合朗缪尔模型，可用于估算丙酮-丁醇-乙醇（ABE）发酵中吸附的正丁醇量。在血清瓶原位吸附发酵中，活性炭表现出最佳性能，可生产 21.9g/L 的正丁醇。在发酵罐中运行时，自由细胞和固定化细胞吸附发酵分别生产 31.6g/L 和 54.6g/L 的正丁醇，相应的生产速率为 0.30g/L·h 和 0.45g/L·h。热解吸产生的冷凝物含有约 167g/L 的正丁醇，在自发相分离后得到约 640g/L 的高浓度正丁醇溶液。这种用活性炭进行的原位产物回收工艺具有节能的特点，并且可以很容易地与 ABE 发酵集成，用于正丁醇的生产。
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