State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
Bioresour Technol. 2014 Jul;164:276-84. doi: 10.1016/j.biortech.2014.04.107. Epub 2014 May 9.
Butanol recovery from acetone-butanol-ethanol (ABE) fed-batch fermentation using permeating-heating-gas was determined in this study. Fermentation was performed with Clostridium acetobutylicum B3 in a fibrous bed bioreactor and permeating-heating-gas stripping was used to eliminate substrate and product inhibition, which normally restrict ABE production and sugar utilization to below 20 g/L and 60 g/L, respectively. In batch fermentation (without permeating-heating-gas stripping), C. acetobutylicum B3 utilized 60 g/L glucose and produced 19.9 g/L ABE and 12 g/L butanol, while in the integrated process 290 g/L glucose was utilized and 106.27 g/L ABE and 66.09 g/L butanol were produced. The intermittent gas stripping process generated a highly concentrated condensate containing approximately 15% (w/v) butanol, 4% (w/v) acetone, a small amount of ethanol (<1%), and almost no acids, resulting in a highly concentrated butanol solution [∼ 70% (w/v)] after phase separation. Butanol removal by permeating-heating-gas stripping has potential for commercial ABE production.
本研究采用渗透加热气体从丙酮-丁醇-乙醇（ABE）分批发酵中回收丁醇。该发酵采用丙酮丁醇梭菌 B3 在纤维床生物反应器中进行，渗透加热气体汽提用于消除底物和产物抑制，通常将 ABE 生产和糖利用率分别限制在 20 g/L 和 60 g/L 以下。在分批发酵（无渗透加热气体汽提）中，C. acetobutylicum B3 利用 60 g/L 葡萄糖，生产 19.9 g/L ABE 和 12 g/L 丁醇，而在集成工艺中，利用 290 g/L 葡萄糖，生产 106.27 g/L ABE 和 66.09 g/L 丁醇。间歇式气体汽提过程产生了一种高浓度的冷凝物，其中含有约 15%（w/v）丁醇、4%（w/v）丙酮、少量乙醇（<1%），且几乎不含酸，经过相分离后得到高浓度的丁醇溶液[约 70%（w/v）]。渗透加热气体汽提去除丁醇有望用于商业 ABE 生产。
