桑黄乙醇提取物可预防二乙基亚硝胺和2-乙酰氨基芴诱导的大鼠肝癌发生。
Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats.
作者信息
Yang Chun-Hung, Chang Heng-Yuan, Chen Yi-Chuan, Lu Chia-Chen, Huang Shyh-Shyun, Huang Guan-Jhong, Lai Hsin-Chih
机构信息
Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Tao-Yuan, 333, Taiwan, Republic of China.
Applied Toxicology Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Executive Yuan, Taichung, Taiwan, 413, China.
出版信息
Chin J Integr Med. 2017 Feb;23(2):117-124. doi: 10.1007/s11655-016-2513-2. Epub 2016 Aug 2.
OBJECTIVE
To study whether the ethanol extract of Phellinus merrillii (EPM) has chemopreventive potential against liver carcinogenesis.
METHODS
Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine (DEN)-initiated, 2-acetylaminofluorene (2-AAF) and partial hepatectomy (PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase (sGOT), glutamic pyruvic transaminase (sGPT) and gamma-glutamyl transpeptidase (γ-GT) were measured.
RESULTS
Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of sGOT, sGPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group (P<0.05 or P<0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity.
CONCLUSION
EPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.
目的
研究黑柄炭角菌乙醇提取物（EPM）对肝癌发生是否具有化学预防潜力。
方法
将30只雄性Spraque-Dawley大鼠随机分为对照组、EPM对照组、肝癌对照组、低剂量EPM组和高剂量EPM组，每组6只。采用Solt和Farber方案建立大鼠肝癌发生模型，评估EPM对二乙基亚硝胺（DEN）启动、2-乙酰氨基芴（2-AAF）和部分肝切除术（PH）促进的大鼠肝癌发生的化学预防作用。进行基本病理生理和组织学检查，并检测血清谷丙转氨酶（sGOT）、谷草转氨酶（sGPT）和γ-谷氨酰转肽酶（γ-GT）水平。
结果
与肝癌对照组相比，在饮食中给予浓度为2 g/kg体重的EPM处理8周，明显预防了癌发生的发展，并降低了大鼠的sGOT、sGPT和血清γ-GT水平（P<0.05或P<0.01）。这些表型伴随着自然杀伤细胞活性的显著增加。
结论
在大鼠模型中，EPM对DEN+2-AAF+PH诱导的肝癌发生显示出强大的肝脏预防作用。
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