利用氢/氘质谱法研究膜蛋白的构象动力学与相互作用
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
作者信息
Forest Eric, Man Petr
机构信息
Univ. Grenoble Alpes, IBS, Grenoble, France.
CNRS, IBS, Grenoble, France.
出版信息
Methods Mol Biol. 2016;1432:269-79. doi: 10.1007/978-1-4939-3637-3_17.
PMID:27485342
Abstract
Hydrogen/deuterium exchange associated with mass spectrometry has been recently used to characterize the dynamics and the interactions of membrane proteins. Here we describe experimental workflow enabling localization of the regions involved in conformational changes or interactions.
摘要
与质谱联用的氢/氘交换技术最近已被用于表征膜蛋白的动力学和相互作用。在此，我们描述了一种实验工作流程，该流程能够定位参与构象变化或相互作用的区域。
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