Cachón Andrés Uc, Quintal-Novelo Carlos, Medina-Escobedo Gilberto, Castro-Aguilar Gaspar, Moo-Puc Rosa E
a Unidad de Investigación Médica Yucatán, Unidad Médica de Alta Especialidad, Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro Social , Mérida , Yucatán , México.
b Departamento de Patología, Unidad Médica de Alta Especialidad, Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro Social , Mérida , Yucatán , México.
J Diet Suppl. 2017 Mar 4;14(2):158-172. doi: 10.1080/19390211.2016.1207003. Epub 2016 Aug 2.
Several studies have shown the hepatoprotective effect of the consumption of coffee and tea, which is mainly attributed to caffeine. Many experimental studies have demonstrated this effect; however, these studies used high caffeine doses that are not related to human consumption. The aim of this study was to evaluate the hepatoprotective effect of low doses of caffeine on carbon tetrachloride (CCl)-treated rats. Low doses of caffeine (CAFF) 5 and 10 mg/kg (CAFF5 and CAFF10) were evaluated in chronic liver damage induced by CCl (0.75 mL/kg) in rats. CAFF treatment was administered once a day and CCl administration was twice weekly for 10 weeks. Liver function tests (biochemical markers) and functional (sleeping time) and histological (hematoxylin-eosin and Masson trichrome stains) parameters were carried out at the end of damage treatment. Daily treatments of CAFF5 and CAFF10 exhibited a hepatoprotective effect supported by a decrease of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (AP) serum activities and bilirubin serum levels compared with control and also restored serum albumin levels and liver glutathione (GSH). Moreover, CAFF prevented CCl-induced prolongation in pentobarbital sleeping time and a decrease of liver fibrosis and cell death. Our results demonstrated that low doses of CAFF exert a hepatoprotective effect against CCl -induced liver damage in rats.
多项研究表明，饮用咖啡和茶具有肝脏保护作用，这主要归因于咖啡因。许多实验研究都证实了这种作用；然而，这些研究使用的咖啡因剂量很高，与人体摄入量无关。本研究的目的是评估低剂量咖啡因对四氯化碳（CCl）处理的大鼠的肝脏保护作用。在大鼠中，对低剂量咖啡因（CAFF）5和10毫克/千克（CAFF5和CAFF10）在由CCl（0.75毫升/千克）诱导的慢性肝损伤中的作用进行了评估。CAFF处理每天给药一次，CCl给药每周两次，持续10周。在损伤处理结束时进行肝功能测试（生化标志物）、功能（睡眠时间）和组织学（苏木精-伊红和马森三色染色）参数检测。与对照组相比，CAFF5和CAFF10的每日处理均表现出肝脏保护作用，表现为丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（AP）血清活性以及胆红素血清水平降低，并且还恢复了血清白蛋白水平和肝脏谷胱甘肽（GSH）。此外，CAFF可防止CCl诱导的戊巴比妥睡眠时间延长以及肝纤维化和细胞死亡的减少。我们的结果表明，低剂量的CAFF对CCl诱导的大鼠肝损伤具有肝脏保护作用。
