Biswas A, Mohan J, Mandal A B, Lal N
Avian Nutrition and Feed Technology Division, ICAR-Central Avian Research Institute, Izatnagar, India.
Avian Physiology and Reproduction Division, ICAR-Central Avian Research Institute, Izatnagar, India.
J Anim Physiol Anim Nutr (Berl). 2017 Apr;101(2):229-235. doi: 10.1111/jpn.12557. Epub 2016 Aug 3.
An attempt was made to investigate the effect of dietary selenium (Se) on physical and cloacal gland size, foam production, biochemical composition of foam and semen biochemical characteristics of male Japanese quail (Coturnix coturnix Japonica). Two hundred twenty-five (225)-day-old male Japanese quail were randomly distributed to three dietary treatment groups for a period of 20 weeks. Each treatment comprised of three replicates, each containing 25 chicks. Three experimental diets were supplemented with 0, 0.5 and 1.0 mg Se/kg (T , T and T respectively), and diet T was considered as control. Sodium selenite was used as the source of selenium. All the birds were provided with feed and water ad libitum. Cloacal foam characteristics, that is cloacal gland index and foam weight, were significantly higher in T group. However, body weight, frequency of foam discharge and testes weight (left and right) did not differ significantly (p > 0.05). Physical characteristics of semen, that is semen volume and sperm concentration, did not differ (p > 0.05) among the Se-treated groups. The sperm motility, live-dead count and abnormality improved significantly (p < 0.05) in 0.5 mg/Se-supplemented group compared to 0 or 1.0 mg/Se-supplemented groups. Similarly, fertility and hatchability percentages were higher (p < 0.05) in 0.5 mg/Se-supplemented group than in control or 1.0 mg/Se-supplemented counterparts. The biochemical characteristics of foam in terms of total protein, acid phosphatase (ACP) and nitric oxide did not differ (p > 0.05), while the concentration of glucose was higher (p < 0.05) in 0.5 mg/Se-supplemented diet. On the other hand, alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were lower (p < 0.05) in 0.5 mg/Se-supplemented group compared to control or 1.0 mg/Se-supplemented groups. From this study, it was concluded that supplementation of 0.5 mg Se/kg diet was beneficial for foam variables, biochemical composition of foam, semen characteristics and fertility in male Japanese quail.
本研究旨在探讨日粮中添加硒（Se）对雄性日本鹌鹑（Coturnix coturnix Japonica）的身体和泄殖腔腺大小、泡沫产生、泡沫生化成分以及精液生化特性的影响。225只日龄雄性日本鹌鹑被随机分为三个日粮处理组，为期20周。每个处理包含三个重复，每个重复有25只雏鸟。三种实验日粮分别添加0、0.5和1.0 mg Se/kg（分别为T₀、T₁和T₂组），T₀组日粮作为对照。亚硒酸钠用作硒源。所有鹌鹑均自由采食和饮水。T₁组的泄殖腔泡沫特征，即泄殖腔腺指数和泡沫重量，显著更高。然而，体重、泡沫排出频率以及睾丸重量（左右）差异不显著（p > 0.05）。硒处理组之间精液的物理特性，即精液体积和精子浓度，差异不显著（p > 0.05）。与添加0或1.0 mg/Se的组相比，添加0.5 mg/Se的组精子活力、死活计数和畸形率显著改善（p < 0.05）。同样，添加0.5 mg/Se的组的受精率和孵化率百分比高于对照组或添加1.0 mg/Se的组（p < 0.05）。泡沫的生化特性，如总蛋白、酸性磷酸酶（ACP）和一氧化氮，差异不显著（p > 0.05），而添加0.5 mg/Se的日粮中葡萄糖浓度更高（p < 0.05）。另一方面，与对照组或添加1.0 mg/Se的组相比，添加0.5 mg/Se的组碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）较低（p < 0.05）。从本研究得出结论，日粮中添加0.5 mg Se/kg对雄性日本鹌鹑的泡沫变量、泡沫生化成分、精液特性和繁殖力有益。
