Xu Jinjia, Takai Atsuro, Takeuchi Masayuki
Molecular Design & Function Group, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, Japan.
Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba, Ibaraki, 305-8571, Japan.
Chemistry. 2016 Sep 5;22(37):13014-8. doi: 10.1002/chem.201602759. Epub 2016 Aug 3.
A red-green-blue (RGB) trichromophoric fluorescent organic nanoparticle exhibiting multi-colour emission was constructed; the blue-emitting cationic oligofluorene nanoparticle acted as an energy-donor scaffold to undergo fluorescence resonance energy transfer (FRET) to a red-emitting dye embedded in the nanoparticle (interior FRET) and to a green-emitting dye adsorbed on the surface through electrostatic interactions (exterior FRET). Each FRET event occurs independently and is free from sequential FRET, thus the resultant dual-FRET system exhibits multi-colour emission, including white, in aqueous solution and film state. A characteristic white-emissive nanoparticle showed visible responses upon perturbation of the exterior FRET efficiency by acceptor displacement, leading to highly sensitive responses toward polyanions in a ratiometric manner. Specifically, our system exhibits high sensitivity toward heparin with an extremely low detection limit.
构建了一种呈现多色发射的红-绿-蓝（RGB）三色荧光有机纳米颗粒；发射蓝光的阳离子低聚芴纳米颗粒作为能量供体支架，通过荧光共振能量转移（FRET）将能量传递给嵌入纳米颗粒内部的发射红光的染料（内部FRET）以及通过静电相互作用吸附在表面的发射绿光的染料（外部FRET）。每个FRET事件独立发生，不存在顺序FRET，因此所得的双FRET系统在水溶液和薄膜状态下均呈现包括白色在内的多色发射。一种具有特征性白色发射的纳米颗粒在通过受体置换扰动外部FRET效率时表现出可见响应，从而以比率方式对多阴离子产生高度敏感的响应。具体而言，我们的系统对肝素具有高灵敏度，检测限极低。
