Liu Yingying, Gao Yunrong, Li Defeng, Fleming Joy, Li Honglin, Bi Lijun
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
National Laboratory of Biomacromolecules and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2016 Aug;72(Pt 8):642-5. doi: 10.1107/S2053230X16010943. Epub 2016 Jul 27.
Rv3899c is a hypothetical protein from Mycobacterium tuberculosis which is conserved across mycobacteria. It is predicted to be secreted and has been found in culture filtrates. It has been proposed as a potential vaccine candidate; however, its biological function is unknown. Here, the global structure of Rv3899c(184-410), a fragment of Rv3899c, is reported. The structure resembles the shell of a sea snail, and its N- and C-termini form two relatively independent compact domains: an α/β/α sandwich folding domain and an α-helix bundle domain. There are no reported protein structures for any Rv3899c homologues; this structure provides the first structural glimpse of a new protein family consisting of Rv3899c and its homologues.
Rv3899c是一种来自结核分枝杆菌的假定蛋白，在分枝杆菌中具有保守性。据预测它会被分泌出来，并且已在培养滤液中被发现。它已被提议作为一种潜在的疫苗候选物；然而，其生物学功能尚不清楚。在此，报道了Rv3899c的一个片段Rv3899c(184 - 410)的整体结构。该结构类似于海螺的外壳，其N端和C端形成两个相对独立的紧密结构域：一个α/β/α三明治折叠结构域和一个α螺旋束结构域。目前尚未有任何Rv3899c同源物的蛋白质结构报道；该结构首次展示了由Rv3899c及其同源物组成的新蛋白质家族的结构。
