Xiao Luosha, Sera Toshihiro, Koshiyama Kenichiro, Wada Shigeo
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Osaka, Japan.
Department of Mechanical Engineering, Faculty of Engineering, Kyushu University, Kyushu, Japan.
Anat Rec (Hoboken). 2016 Oct;299(10):1424-34. doi: 10.1002/ar.23452. Epub 2016 Aug 12.
Understanding the three-dimensional morphology of pulmonary acini is essential when exploring the biomechanics of respiratory function. In this study, we characterized the morphology of individual acini and a cluster of acini stemming from the same terminal conducting airway using a quantitative approach based on the semi-automatic segmentation of synchrotron micro-CT images of mouse lung. The volume and surface area of five clusters of mouse acini including 50 individual acini were estimated based on the voxel and surface mesh of segmented acini at FRC. The pathway length and width were estimated for one cluster including 15 acini based on the skeleton of segmented acini. The acinar volume was 0.09 ± 0.07 mm(3) (mean ± SD), and the surface area was 6.82 ± 4.49 mm(2) , in agreement with previous studies. The volume of the acinar clusters was 0.89 ± 0.34 mm(3) , and the surface area was 68.18 ± 17.66 mm(2) . The largest volume acinus per cluster was found in the distal region of the terminal conducting airway, and apparent respiratory bronchioles were observed only in large-volume acini. The generation number of pathways per acinus was 8 ± 2 (range: 6-12). The pathway length at lower generations (generations 2-6) increased with the generation number in a single cluster, while did not significantly change at lower generations in some acinar groups. The pathway width increased with increasing generation numbers. Our approach characterized the quantitative morphology of pulmonary acinar clusters in mouse lung, and the results can be used in further biomechanical simulation studies. Anat Rec, 299:1424-1434, 2016. © 2016 Wiley Periodicals, Inc.
在探究呼吸功能的生物力学时，了解肺腺泡的三维形态至关重要。在本研究中，我们基于对小鼠肺同步辐射显微CT图像的半自动分割，采用定量方法对源自同一终末传导气道的单个腺泡和腺泡簇的形态进行了表征。基于FRC时分割腺泡的体素和表面网格，估计了包括50个单个腺泡的五组小鼠腺泡的体积和表面积。基于分割腺泡的骨架，估计了包括15个腺泡的一组腺泡的路径长度和宽度。腺泡体积为0.09±0.07mm³（平均值±标准差），表面积为6.82±4.49mm²，与先前研究一致。腺泡簇的体积为0.89±0.34mm³，表面积为68.18±17.66mm²。每组中最大体积的腺泡位于终末传导气道的远端区域，仅在大体积腺泡中观察到明显的呼吸性细支气管。每个腺泡的路径生成数为8±2（范围：6 - 12）。在单个腺泡簇中，较低代（第2 - 6代）的路径长度随代数增加而增加，而在一些腺泡组中较低代时路径长度无显著变化。路径宽度随代数增加而增加。我们的方法表征了小鼠肺中肺腺泡簇的定量形态，其结果可用于进一步的生物力学模拟研究。《解剖学记录》，299:1424 - 1434，2016年。©2016威利期刊公司。
