Niu Li-Na, Pei Dan-Dan, Morris Matthew, Jiao Kai, Huang Xue-Qing, Primus Carolyn M, Susin Lisiane F, Bergeron Brian E, Pashley David H, Tay Franklin R
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China; Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
Department of Prosthodontics, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
Dent Mater. 2016 Oct;32(10):1235-1247. doi: 10.1016/j.dental.2016.07.007. Epub 2016 Aug 3.
An experimental discoloration-free calcium aluminosilicate cement has been developed with the intention of maximizing the beneficial attributes of tricalcium silicate cements and calcium aluminate cements. The present study examined the effects of this experimental cement (Quick-Set2) on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available tricalcium silicate cement (white mineral trioxide aggregate (ProRoot(®) MTA); WMTA).
The osteogenic potential of hDPSCs exposed to the cements was examined using qRT-PCR for osteogenic gene expressions, Western blot for osteogenic-related protein expressions, alkaline phosphatase enzyme activity, Alizarin red S staining, Fourier transform infrared spectroscopy and transmission electron microscopy of extracellular calcium deposits.
Results of the six assays indicated that osteogenic differentiation of hDPSCs was significantly enhanced after exposure to the tricalcium silicate cement or the experimental calcium aluminosilicate cement, with the former demonstrating better mineralogenic stimulation capacity.
The better osteogenic stimulating effect of the tricalcium silicate cement on hDPSCs may be due to its relatively higher silicate content, or higher OH(-) and Ca(2+) release. Further investigations with the use of in vivo animal models are required to validate the potential augmenting osteogenic effects of the experimental discoloration-free calcium aluminosilicate cement.
已研发出一种实验性无变色硅酸钙铝水泥，旨在最大化硅酸三钙水泥和铝酸钙水泥的有益特性。本研究通过将细胞反应与市售硅酸三钙水泥（白色矿物三氧化物凝聚体（ProRoot® MTA）；WMTA）进行比较，考察了这种实验性水泥（Quick-Set2）对成骨谱系定向人牙髓干细胞（hDPSCs）矿化特性的影响。
使用qRT-PCR检测成骨基因表达、Western blot检测成骨相关蛋白表达、碱性磷酸酶活性、茜素红S染色、傅里叶变换红外光谱以及细胞外钙沉积的透射电子显微镜，来检测暴露于这些水泥的hDPSCs的成骨潜力。
六项检测结果表明，hDPSCs暴露于硅酸三钙水泥或实验性硅酸钙铝水泥后，其成骨分化显著增强，前者表现出更好的矿化刺激能力。
硅酸三钙水泥对hDPSCs具有更好的成骨刺激作用，可能是由于其相对较高的硅酸盐含量，或较高的OH(-)和Ca(2+)释放量。需要使用体内动物模型进行进一步研究，以验证实验性无变色硅酸钙铝水泥潜在的增强成骨作用。
