构建嵌入细胞外基质的三维上皮组织。
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix.
作者信息
Piotrowski-Daspit Alexandra S, Nelson Celeste M
机构信息
Chemical and Biological Engineering, Princeton University.
Chemical and Biological Engineering, Princeton University; Molecular Biology, Princeton University;
出版信息
J Vis Exp. 2016 Jul 10(113):54283. doi: 10.3791/54283.
The architecture of branched organs such as the lungs, kidneys, and mammary glands arises through the developmental process of branching morphogenesis, which is regulated by a variety of soluble and physical signals in the microenvironment. Described here is a method created to study the process of branching morphogenesis by forming engineered three-dimensional (3D) epithelial tissues of defined shape and size that are completely embedded within an extracellular matrix (ECM). This method enables the formation of arrays of identical tissues and enables the control of a variety of environmental factors, including tissue geometry, spacing, and ECM composition. This method can also be combined with widely used techniques such as traction force microscopy (TFM) to gain more information about the interactions between cells and their surrounding ECM. The protocol can be used to investigate a variety of cell and tissue processes beyond branching morphogenesis, including cancer invasion.
诸如肺、肾和乳腺等分支器官的结构是通过分支形态发生的发育过程形成的，该过程受微环境中多种可溶性和物理信号的调节。本文描述了一种创建的方法，用于通过形成完全嵌入细胞外基质（ECM）中的具有确定形状和大小的工程化三维（3D）上皮组织来研究分支形态发生过程。这种方法能够形成相同组织的阵列，并能够控制多种环境因素，包括组织几何形状、间距和ECM组成。该方法还可以与广泛使用的技术（如牵引力显微镜（TFM））相结合，以获取更多关于细胞与其周围ECM之间相互作用的信息。该方案可用于研究分支形态发生以外的各种细胞和组织过程，包括癌症侵袭。
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