Dannhauser David, Rossi Domenico, Memmolo Pasquale, Causa Filippo, Finizio Andrea, Ferraro Pietro, Netti Paolo A
Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT), Largo Barsanti e Matteucci 53, 80125, Naples, Italy.
CNR-ISASI Institute of Applied Sciences & Intelligent Systems "E. Caianiello", Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
J Biophotonics. 2017 May;10(5):683-689. doi: 10.1002/jbio.201600070. Epub 2016 Aug 9.
The investigation of the physical properties of peripheral blood mononuclear cells (PBMC) is of great relevance, as they play a key role in regulating human body health. Here we report the possibility to characterize human PBMC in their physiological conditions in a microfluidic-based measurement system. A viscoelastic polymer solution is adopted for 3D alignment of individual cells inflow. An optical signature (OS) acquisition of each flowing cell is performed using a wide angle light scattering apparatus. Besides, a quantitative phase imaging (QPI) holographic system is employed with the aim (i) to check the position in flow of individual cells using a holographic 3D cell tracking method; and (ii) to estimate their 3D morphometric features, such as their refractive index (RI). Results obtained by combining OS and QPI have been compared with literature values, showing good agreement. The results confirm the possibility to obtain sub-micrometric details of physical cell properties in microfluidic flow, avoiding chemical staining or fluorescent labelling.
对外周血单个核细胞（PBMC）物理特性的研究具有重要意义，因为它们在调节人体健康方面起着关键作用。在此，我们报告了在基于微流体的测量系统中对处于生理状态的人PBMC进行表征的可能性。采用粘弹性聚合物溶液对单个细胞流入进行三维排列。使用广角光散射装置对每个流动细胞进行光学特征（OS）采集。此外，采用定量相成像（QPI）全息系统，目的是：（i）使用全息三维细胞跟踪方法检查单个细胞在流动中的位置；（ii）估计它们的三维形态特征，如折射率（RI）。将OS和QPI相结合获得的结果与文献值进行了比较，显示出良好的一致性。结果证实了在微流体流动中获得细胞物理特性亚微米级细节的可能性，避免了化学染色或荧光标记。
