Czerny C P, Mahnel H, Hornstein O
Zentralbl Veterinarmed B. 1989 Mar;36(2):100-12.
An infection model was developed, which allows the study of humoral and cellular immune response mechanisms induced by Orthopox viruses in mice. The optimal infection route for neurovirulent vaccinia virus strains was investigated and resulted in well-defined clinical symptoms in non-immunized susceptible mice. Signs of disease were taken as a basis for comparison. Challenge infections by intracutaneous (i.c.) and intraperitoneal (i.p.) application of vaccinia strain Munich 1 (M1) appeared to be most suited to testing immunities of different status in vivo. Mice passively immunized with an anti-vaccinia immune serum survived intraperitoneal challenge infection with 4LD50/mouse. After an intracutaneous challenge infection with 10(4) TCID50/animal, however, they were fully susceptible. Mice immunized with live vaccinia virus showed a solid immunity to both intracutaneous and intraperitoneal challenge.
建立了一种感染模型，可用于研究正痘病毒在小鼠体内诱导的体液免疫和细胞免疫反应机制。研究了神经毒性痘苗病毒株的最佳感染途径，并在未免疫的易感小鼠中产生了明确的临床症状。以疾病症状作为比较的基础。通过皮内（i.c.）和腹腔内（i.p.）接种痘苗株慕尼黑1（M1）进行的攻击感染似乎最适合在体内测试不同状态的免疫力。用抗痘苗免疫血清进行被动免疫的小鼠在腹腔内接受4LD50/小鼠的攻击感染后存活。然而，在皮内接受10(4) TCID50/动物的攻击感染后，它们完全易感。用活痘苗病毒免疫的小鼠对皮内和腹腔内攻击均表现出牢固的免疫力。
