Shin Hyoseung, Yoon Ji-Seon, Koh Wooseok, Kim Jin Yong, Kim Chang-Hyun, Han Kang Min, Kim Eo Jin, Kwon Ohsang
Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Korea.
Department of Dermatology, Institute of Human-Environmental Interface Biology, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
Lasers Surg Med. 2016 Oct;48(8):748-762. doi: 10.1002/lsm.22570. Epub 2016 Aug 9.
Lasers have been successfully used for decades to remove dark hair. However, laser removal of nonpigmented hair is challenging due to the lack of chromophores. The aim of this study was to use photodynamic therapy (PDT) to remove nonpigmented hair.
STUDY DESIGN/MATERIAL AND METHODS: We compared the efficacy of permanent hair reduction in white BALB/c and black C57BL/6 mice treated with PDT or an 800-nm diode laser. We collected skin biopsy specimens and investigated post-PDT histologic changes and molecular changes.
We observed keratin 15 staining in the bulge area and alkaline phosphatase staining in the dermal papilla following PDT. We observed a temporary, catagen-like transformation in nonpigmented hair follicles after PDT. We observed apoptotic cells in the hair matrix after PDT. Irradiation with an 800-nm diode laser did not achieve nonpigmented hair removal. Multiple PDT sessions achieved permanent reduction of nonpigmented hair. Interestingly, removal of black hair using PDT was less efficient.
Our results suggest that PDT can damage the nonpigmented hair matrix, but not stem cells or dermal papillae. Repeated PDT may impair the hair-regeneration capacity via a bystander effect on bulge stem cells or dermal papillae. In this study, we found it was possible to remove nonpigmented hair using PDT. Lasers Surg. Med. 48:748-762, 2016. © 2016 Wiley Periodicals, Inc.
几十年来，激光已成功用于去除深色毛发。然而，由于缺乏发色团，激光去除无色素毛发具有挑战性。本研究的目的是使用光动力疗法（PDT）去除无色素毛发。
研究设计/材料与方法：我们比较了接受PDT或800纳米二极管激光治疗的白色BALB/c小鼠和黑色C57BL/6小鼠永久性减少毛发的效果。我们收集了皮肤活检标本，并研究了PDT后的组织学变化和分子变化。
PDT后，我们观察到隆突区有角蛋白15染色，真皮乳头有碱性磷酸酶染色。PDT后，我们观察到无色素毛囊出现暂时的、类似退行期的转变。PDT后，我们在毛基质中观察到凋亡细胞。800纳米二极管激光照射未能实现无色素毛发的去除。多次PDT治疗可实现无色素毛发的永久性减少。有趣的是，使用PDT去除黑色毛发的效率较低。
我们的结果表明，PDT可损伤无色素毛基质，但不会损伤干细胞或真皮乳头。重复PDT可能通过对隆突干细胞或真皮乳头的旁观者效应损害毛发再生能力。在本研究中，我们发现使用PDT去除无色素毛发是可能的。《激光外科与医学》48:748 - 762，2016年。©2016威利期刊公司。
