2,3,7,8-四氯二苯并对二恶英通过小鼠胚胎腭间充质细胞介导腭裂

2,3,7,8-Tetrachlorodibenzo-p-dioxin Mediated Cleft palate by Mouse Embryonic Palate Mesenchymal Cells.

作者信息

Gao Liyun, Wang Yan, Yao Yongcheng, Zhang Guanghui, Han Guangliang, Wu Weidong

机构信息

School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, Henan, 453003, China; Department of Toxicology, College of Public Health, Zhengzhou University, 100 Science Road, Zhengzhou, Henan, 450001, China.

School of Life Sciences and Technology, Xinxiang Medical University, 601 Jinsui Avenue, Xinxiang 453003, China.

出版信息

Arch Oral Biol. 2016 Nov;71:150-154. doi: 10.1016/j.archoralbio.2016.08.002. Epub 2016 Aug 2.

DOI:10.1016/j.archoralbio.2016.08.002

PMID:27505035

Abstract

OBJECTIVE

To evaluate the effects of mouse embryonic palatal mesenchymal (MEPM) cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced.

STUDY DESIGN

An experimental study at Xinxiang Medical University.

METHODS

Primary MEPM cells were derived from palatal tissue on 30 pregnant C57BL/6 female mice (embryonic day13, GD13). The MEPM cells were placed in a humidified incubator at 37°C with 5% CO atmosphere with media replaced every other day. The third passage cells were seeded, and one part of cells were treated with 10nM TCDD (TCDD group). And others were treated with DMSO (≤0.05%, as control group). After 72h, MTT assay was analysed cell viability. Scratch wound-healing was analysed cell motility. Flow cytometry was analysed cell apoptosis and cycle. Western blot was analysed the expression of cyclinE, CDK2, P16 and P21.

RESULTS

TCDD inhibited the growth and migration of MEPM cells, while increased cell apoptosis. TCDD exposure inhibited the progression of cells from G1 to S phase and tended to reduce the number of cells entering the G2/M phase. TCDD inhibited expression of cyclinE and CDK2 at the protein level, instead increased the expression of P16 and p21 proteins.

CONCLUSIONS

TCDD might induce cleft palate by altering MEPM cells.

摘要

目的

评估2,3,7,8-四氯二苯并对二恶英（TCDD）诱导小鼠胚胎腭间充质（MEPM）细胞产生的影响。

研究设计

在新乡医学院进行的一项实验研究。

方法

从30只怀孕的C57BL/6雌性小鼠（胚胎第13天，妊娠第13天）的腭组织中获取原代MEPM细胞。将MEPM细胞置于37°C、含5%二氧化碳的湿润培养箱中，每隔一天更换培养基。接种第三代细胞，一部分细胞用10nM TCDD处理（TCDD组），另一部分用二甲基亚砜（≤0.05%，作为对照组）处理。72小时后，采用MTT法分析细胞活力，划痕伤口愈合实验分析细胞迁移能力，流式细胞术分析细胞凋亡和细胞周期，蛋白质免疫印迹法分析细胞周期蛋白E、细胞周期蛋白依赖性激酶2、P16和P21的表达。