Xu Jiaoyan, Cao Xiyue, Xia Jianfei, Gong Shida, Wang Zonghua, Lu Lin
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, PR China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, PR China.
Anal Chim Acta. 2016 Aug 31;934:44-51. doi: 10.1016/j.aca.2016.06.033. Epub 2016 Jun 22.
A sensitive non-enzymatic glucose electrochemical biosensor (Cu/PMo12-GR/GCE) was developed based on the combination of copper nanoparticles (CuNPs) and phosphomolybdic acid functionalized graphene (PMo12-GR). PMo12-GR films were modified on the surface of glassy carbon electrode (GCE) through electrostatic self-assembly with the aid of poly diallyl dimethyl ammonium chloride (PDDA). Then CuNPs were successfully decorated onto the PMo12-GR modified GCE through electrodeposition. The morphology of Cu/PMo12-GR/GCE was characterized by scanning electron microscope (SEM). Cyclic voltammetry (CV) and chronoamperometry were used to investigate the electrochemical performances of the biosensor. The results indicated that the modified electrode displayed a synergistic effect of PMo12-GR sheets and CuNPs towards the electro-oxidation of glucose in the alkaline solution. At the optimal detection potential of 0.50 V, the response towards glucose presented a linear response ranging from 0.10 μM to 1.0 mM with a detection limit of 3.0 × 10(-2) μM (S/N = 3). In addition, Cu/PMo12-GR/GCE possessed a high selectivity, good reproducibility, excellent stability and acceptable recovery, which indicating the potential application in clinical field.
基于铜纳米颗粒（CuNPs）和磷钼酸功能化石墨烯（PMo12-GR）的结合，开发了一种灵敏的非酶葡萄糖电化学 biosensor（Cu/PMo12-GR/GCE）。借助聚二烯丙基二甲基氯化铵（PDDA）通过静电自组装在玻碳电极（GCE）表面修饰 PMo12-GR 薄膜。然后通过电沉积将 CuNPs 成功修饰到 PMo12-GR 修饰的 GCE 上。通过扫描电子显微镜（SEM）对 Cu/PMo12-GR/GCE 的形态进行了表征。采用循环伏安法（CV）和计时电流法研究了该 biosensor 的电化学性能。结果表明，修饰电极在碱性溶液中对葡萄糖的电氧化表现出 PMo12-GR 片层和 CuNPs 的协同作用。在 0.50 V 的最佳检测电位下，对葡萄糖的响应呈现出从 0.10 μM 到 1.0 mM 的线性响应，检测限为 3.0×10(-2) μM（S/N = 3）。此外，Cu/PMo12-GR/GCE 具有高选择性、良好的重现性、优异的稳定性和可接受的回收率，这表明其在临床领域具有潜在的应用价值。
