用于基于辣根过氧化物酶检测的纸质装置上的3,3',5,5'-四甲基联苯胺氧化反应
3,3',5,5'-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays.
作者信息
Busa Lori Shayne Alamo, Komatsu Takeshi, Mohammadi Saeed, Maeki Masatoshi, Ishida Akihiko, Tani Hirofumi, Tokeshi Manabu
机构信息
Graduate School of Chemical Sciences and Engineering, Hokkaido University.
出版信息
Anal Sci. 2016;32(8):815-8. doi: 10.2116/analsci.32.815.
We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration.
我们报道了在光刻制造的（P-PAD）和蜡印的（W-PAD）纸基分析装置上，使用辣根过氧化物酶通过过氧化氢对3,3',5,5'-四甲基联苯胺（TMB）进行比色氧化。通过光刻制造PAD会使亲水区域暴露于聚合物（光刻胶）和溶剂中，这不仅会降低亲水性，还会影响TMB-H2O2检测系统，因为在制造过程中光刻胶不可避免地无法完全消除。然后，即使在空白HRP浓度下，在P-PAD上存在光刻胶残留的情况下也能观察到检测信号。
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