从FASTQ到功能：处理下一代测序数据的计算机方法

From FASTQ to Function: In Silico Methods for Processing Next-Generation Sequencing Data.

作者信息

Preston Mark D, Stabler Richard A

机构信息

National Institute for Biological Standards and Control, South Mimms, UK.

Faculty of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK.

出版信息

Methods Mol Biol. 2016;1476:23-33. doi: 10.1007/978-1-4939-6361-4_3.

DOI:10.1007/978-1-4939-6361-4_3

PMID:27507331

Abstract

This chapter presents a method to process C. difficile whole-genome next-generation sequencing data straight from the sequencer. Quality control processing and de novo assembly of these data enable downstream analyses such as gene annotation and in silico multi-locus strain-type identification.

摘要

本章介绍了一种直接处理来自测序仪的艰难梭菌全基因组二代测序数据的方法。对这些数据进行质量控制处理和从头组装，可实现下游分析，如基因注释和计算机多位点菌株类型鉴定。