Crowther Grace S, Wilcox Mark H, Chilton Caroline H
Healthcare Associated Infection Research Group (Leeds Institute for Biomedical and Clinical Sciences), Old Medical School, Leeds General Infirmary, University of Leeds, Leeds, LS1 3EX, West Yorkshire, UK.
Leeds Teaching Hospitals NHS Trust, Microbiology Department, Old Medical School, Leeds General Infirmary, Leeds, LS1 3EX, West Yorkshire, UK.
Methods Mol Biol. 2016;1476:223-34. doi: 10.1007/978-1-4939-6361-4_17.
The in vitro gut model is an invaluable research tool to study indigenous gut microbiota communities, the behavior of pathogenic organisms, and the therapeutic and adverse effect of antimicrobial administration on these communities. The model has been validated against the intestinal contents of sudden death victims to reflect the physicochemical and microbiological conditions of the proximal to distal colon, and has been extensively used to investigate the interplay between gut microbiota populations, antibiotic exposure, and Clostridium difficile infection. More recently the gut model has been adapted to additionally model intestinal biofilm. Here we describe the structure, assembly, and application of the biofilm gut model.
体外肠道模型是研究肠道原生微生物群落、致病生物行为以及抗菌药物给药对这些群落的治疗和不良反应的宝贵研究工具。该模型已针对猝死受害者的肠道内容物进行了验证，以反映近端至远端结肠的物理化学和微生物学状况，并已广泛用于研究肠道微生物群、抗生素暴露和艰难梭菌感染之间的相互作用。最近，肠道模型已被调整用于额外模拟肠道生物膜。在此，我们描述生物膜肠道模型的结构、组装和应用。
