类风湿关节炎患者软骨下骨髓微环境中辅助性T细胞17和辅助性T细胞1增加，但调节性T细胞减少。
T helper 17 and T helper 1 cells are increased but regulatory T cells are decreased in subchondral bone marrow microenvironment of patients with rheumatoid arthritis.
作者信息
Wang Ting, Li Shufeng, Yang Yun, Zhang Kaining, Dong Shixiao, Wang Xiuhua, Liu Xinguang, Ren Yanjun, Zhang Ming, Yan Xinfeng, Li Jianmin, Zhang Lei
机构信息
Department of Obstetrics and Gynecology, Shandong Provincial Qianfoshan Hospital, Shandong University Jinan 250014, P.R. China.
Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Shandong University Jinan 250014, P.R. China.
出版信息
Am J Transl Res. 2016 Jul 15;8(7):2956-68. eCollection 2016.
OBJECTIVES
The present study is to investigate the profiles of Th17, Th1 and Treg cells in bone marrow of patients with rheumatoid arthritis (RA).
METHODS
Flow cytometry was used to analyze the frequencies of Th17, Th1 and Treg cells in paired peripheral blood and bone marrow of 26 RA patients and 11 osteoarthritis (OA) patients, as well as 10 healthy controls. In addition, the disease activity was analyzed by the 28-joint disease activity score (DAS28).
RESULTS
The frequencies of Th17 and Th1 cells were significantly elevated in bone marrow of RA patients. Importantly, Th17 and Th1 cells were significantly elevated in bone marrow compared with the matched peripheral blood from RA patients. However, Treg cells were significantly decreased in bone marrow of RA patients compared with the matched peripheral blood of RA patients and bone marrow of osteoarthritis patients and healthy controls. Moreover, the frequencies of tumor necrosis factor-α-producing T cells were significantly elevated in bone marrow from RA patients. Additionally, Th17 and Th1 cells in bone marrow were positively correlated with DAS28, while Treg cells were negatively correlated with DAS28.
CONCLUSIONS
The present study demonstrates that Th17 and Th1 cells are markedly increased in bone marrow from RA patients. By contrast, Treg cells are significantly decreased in bone marrow from RA patients. These results suggest that local abnormality of Th17, Th1 and Treg cells in bone marrow of RA patients may contribute to bone destruction in skeletal system.
目的
本研究旨在调查类风湿关节炎（RA）患者骨髓中Th17、Th1和调节性T细胞（Treg）的特征。
方法
采用流式细胞术分析26例RA患者、11例骨关节炎（OA）患者以及10名健康对照者配对的外周血和骨髓中Th17、Th1和Treg细胞的频率。此外，通过28关节疾病活动评分（DAS28）分析疾病活动度。
结果
RA患者骨髓中Th17和Th1细胞的频率显著升高。重要的是，与RA患者匹配的外周血相比，骨髓中的Th17和Th1细胞显著升高。然而，与RA患者匹配的外周血、骨关节炎患者的骨髓以及健康对照者相比，RA患者骨髓中的Treg细胞显著减少。此外，RA患者骨髓中产生肿瘤坏死因子-α的T细胞频率显著升高。另外，骨髓中的Th17和Th1细胞与DAS28呈正相关，而Treg细胞与DAS28呈负相关。
结论
本研究表明，RA患者骨髓中Th17和Th1细胞明显增加。相比之下，RA患者骨髓中的Treg细胞显著减少。这些结果表明，RA患者骨髓中Th17、Th1和Treg细胞的局部异常可能导致骨骼系统的骨质破坏。
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