Hong Xin, Wang Xiaofeng, Wang Tian, Yu Chengfan, Li Hui
Department of Urology, Peking University International Hospital Beijing 102206, China.
Am J Transl Res. 2016 Jul 15;8(7):3227-34. eCollection 2016.
This study aimed to investigate the nanobacteria (NB) induced damage to human tubular epithelial HK-2 cells and the potential role of NB in the kidney stone formation.
Serum sample from 15 patients with kidney stone was collected. Four groups were included: control, NB group, nanograde hydroxyapatite (nHAP) and calcium oxalate monohydrate (COM) group. Catalase (CAT), malonaldehyde (MDA) and Na(+)/K(+) ATPase activity was detected in the supernatant at 12 and 24 h. At 12 and 24 h, COM was added.
At 12 h and 24 h, the CAT in NB group was significantly higher than in control group and nHAP group (P<0.01). CAT at 24 h was significantly higher than in COM group (P<0.01). At 12 h and 24 h, the MDA in NB group was significantly higher than in control group and nHAP group (P<0.01) and significantly lower than in COM group (P<0.01). At 12 h, the Na(+)/K(+) ATPase activity in NB group and nHAP group was significantly lower than in control group, but dramatically increased as compared to COM group (P<0.01). At 24 h, the Na(+)/K(+) ATPase activity in NB group and nHAP group was significantly lower than in control group (P<0.01).
NB may induce lipid peroxidation in HK-2 cells and cause adhesion of HK-2 cells to COM in a time-dependent manner, resulting in damage to HK-2 cells. This injury-causing capability of NB is more potent than nHAP and might be involved in the pathogenesis of kidney stone formation.
本研究旨在探讨纳米细菌（NB）对人肾小管上皮HK - 2细胞的损伤作用以及NB在肾结石形成中的潜在作用。
收集15例肾结石患者的血清样本。分为四组：对照组、NB组、纳米级羟基磷灰石（nHAP）组和一水合草酸钙（COM）组。在12小时和24小时时检测上清液中的过氧化氢酶（CAT）、丙二醛（MDA）和Na(+)/K(+) ATP酶活性。在12小时和24小时时加入COM。
在12小时和24小时时，NB组的CAT显著高于对照组和nHAP组（P<0.01）。24小时时的CAT显著高于COM组（P<0.01）。在12小时和24小时时，NB组的MDA显著高于对照组和nHAP组（P<0.01），且显著低于COM组（P<0.01）。在12小时时，NB组和nHAP组的Na(+)/K(+) ATP酶活性显著低于对照组，但与COM组相比显著升高（P<0.01）。在24小时时，NB组和nHAP组的Na(+)/K(+) ATP酶活性显著低于对照组（P<0.01）。
NB可能诱导HK - 2细胞发生脂质过氧化，并以时间依赖性方式导致HK - 2细胞与COM黏附，从而对HK - 2细胞造成损伤。NB的这种致伤能力比nHAP更强，可能参与了肾结石形成的发病机制。
