血细胞比容对血小板黏附的影响：实验与模拟
The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations.
作者信息
Spann Andrew P, Campbell James E, Fitzgibbon Sean R, Rodriguez Armando, Cap Andrew P, Blackbourne Lorne H, Shaqfeh Eric S G
机构信息
Department of Chemical Engineering, University of Texas at Austin, Austin, Texas.
Schertz, Texas 78154, USA.
出版信息
Biophys J. 2016 Aug 9;111(3):577-588. doi: 10.1016/j.bpj.2016.06.024.
Abstract
The volume fraction of red blood cells (RBCs) in a capillary affects the degree to which platelets are promoted to marginate to near a vessel wall and form blood clots. In this work we investigate the relationship between RBC hematocrit and platelet adhesion activity. We perform experiments flowing blood samples through a microfluidic channel coated with type 1 collagen and observe the rate at which platelets adhere to the wall. We compare these results with three-dimensional boundary integral simulations of a suspension of RBCs and platelets in a periodic channel where platelets can adhere to the wall. In both cases, we find that the rate of platelet adhesion varies greatly with the RBC hematocrit. We observe that the relative decrease in platelet activity as hematocrit falls shows a similar profile for simulation and experiment.
摘要
毛细血管中红细胞（RBC）的体积分数会影响血小板被促进边缘化至血管壁附近并形成血凝块的程度。在这项工作中，我们研究了红细胞压积与血小板黏附活性之间的关系。我们进行了让血液样本流经涂有I型胶原蛋白的微流控通道的实验，并观察血小板黏附到壁上的速率。我们将这些结果与红细胞和血小板悬浮液在周期性通道中的三维边界积分模拟结果进行比较，在该周期性通道中血小板可以黏附到壁上。在这两种情况下，我们都发现血小板黏附速率随红细胞压积变化很大。我们观察到，随着红细胞压积下降，血小板活性的相对降低在模拟和实验中呈现出相似的趋势。
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