印度西部浦那接种轮状病毒疫苗儿童中的轮状病毒感染情况

Rotavirus Infections in Children Vaccinated Against Rotavirus in Pune, Western India.

作者信息

Jain Preeti, Varanasi Gopalkrishna, Ghuge Rohan, Kalrao Vijay, Dhongade Ram, Bavdekar Ashish, Mehendale Sanjay, Chitambar Shobha

机构信息

Enteric Viruses Group, National Institute of Virology, Pune; *Bharati Vidyapeeth Medical College and Hospital, Pune; Sant Dnyaneshwar Medical Foundation and Research Centres Shaishav Clinic, Pune; King Edward Memorial Hospital, Pune; and National Institute of Epidemiology, Chennai; India. Correspondence to: Dr Shobha D Chitambar, Enteric Viruses Group, National Institute of Virology, 20A, Ambedkar Road, PO Box No 11, Pune 411 001, India.

出版信息

Indian Pediatr. 2016 Jul 8;53(7):589-93. doi: 10.1007/s13312-016-0893-1.

DOI:10.1007/s13312-016-0893-1

PMID:27508535

Abstract

OBJECTIVE

To characterize rotavirus infections detected in rotavirus vaccinated children hospitalized for acute gastroenteritis.

DESIGN

Observational, hospital-based study.

SETTING

Three hospitals in Pune, Western India.

PARTICIPANTS

Children aged <5 years hospitalized for acute gastroenteritis during 2013-14.

METHODS

Rotavirus capture ELISA was performed on all stool samples that were collected from patients following informed consent from parents. VP7 and VP4 genes of rotavirus strains were genotyped by multiplex RT-PCR. Stool samples from vaccinated children were tested for other enteric viruses.

RESULTS

Among the 529 children, 53 were vaccinated with at least one dose of the rotavirus vaccine. There was no difference in the mean (SD) (months) age of vaccinated [14.8 (10.6)] and unvaccinated [14.4 (10.5)] children. Rotavirus positivity was significantly higher (47%) in unvaccinated than in vaccinated (28.3%) children (P=0.01). Mean Vesikari score and severe cases were significantly more in rotavirus positive than in negative children within unvaccinated group (P<0.001), while these did not differ within the vaccinated group. Rotavirus strain G1P[8] was identified as the most prevalent strain in both, vaccinated (60%) and unvaccinated (72.8%) groups. No association was found between mean Vesikari score and viral coinfections.

CONCLUSIONS

This study suggests decline in rotavirus positivity in rotavirus-vaccinated children hospitalized for acute gastroenteritis and high prevalence of G1P[8] and non-rotaviral co-infections in Pune, Western India.

摘要

目的

对因急性胃肠炎住院的接种轮状病毒疫苗儿童中检测到的轮状病毒感染进行特征分析。

设计

基于医院的观察性研究。

地点

印度西部浦那的三家医院。

参与者

2013 - 2014年因急性胃肠炎住院的5岁以下儿童。

方法

在获得家长知情同意后，对所有从患者收集的粪便样本进行轮状病毒捕获ELISA检测。通过多重RT - PCR对轮状病毒株的VP7和VP4基因进行基因分型。对接种疫苗儿童的粪便样本检测其他肠道病毒。

结果

在529名儿童中，53名至少接种了一剂轮状病毒疫苗。接种疫苗儿童[14.8(10.6)]和未接种疫苗儿童[14.4(10.5)]的平均（标准差）年龄无差异。未接种疫苗儿童的轮状病毒阳性率（47%）显著高于接种疫苗儿童（28.3%）（P = 0.01）。在未接种疫苗组中，轮状病毒阳性儿童的平均维西卡里评分和重症病例显著多于阴性儿童（P < 0.001），而在接种疫苗组中则无差异。轮状病毒株G1P[8]被确定为接种疫苗组（60%）和未接种疫苗组（72.8%）中最常见的毒株。未发现平均维西卡里评分与病毒合并感染之间存在关联。