Podolak-Popinigis Justyna, Ronowicz Anna, Dmochowska Monika, Jakubiak Agnieszka, Sachadyn Paweł
Department of Molecular Biotechnology & Microbiology, Gdańsk University of Technology, Gdańsk, Poland.
Department of Biology & Pharmaceutical Botany, Medical University of Gdańsk, Gdańsk, Poland.
Epigenomics. 2016 Oct;8(10):1331-1345. doi: 10.2217/epi-2016-0068. Epub 2016 Aug 11.
Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition.
MATERIALS & METHODS: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach.
RESULTS & CONCLUSION: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after. A number of the methylome alterations were retained until adulthood and correlated with gene expression, while the functional associations imply that scarless healing depends on epigenetic regulation.
已知胎儿皮肤愈合无瘢痕。在小鼠中，这种现象在妊娠第16 - 17天之前都能观察到——这是从无瘢痕愈合转变为正常愈合的日子。本研究旨在确定转变后关键的甲基化组和转录组变化。
采用微阵列方法分析小鼠背部皮肤的甲基化组和转录组图谱。
与炎症反应和透明质酸降解相关的基因在转变前DNA甲基化增加，而参与胚胎形态发生、神经元分化和突触功能的基因在转变后DNA甲基化增加。许多甲基化组改变一直保留到成年并与基因表达相关，而功能关联表明无瘢痕愈合依赖于表观遗传调控。
