Barros Hélio L, Marques Sandra M T, Stefani Valter
Institute of Chemistry, Laboratory of New Organic Materials and Forensic Chemistry (LNMO-QF), Universidade Federal do Rio Grande do Sul (UFRGS). Av. Bento Gonçalves, 9.500, C.P. 15003, 91.501-970 Porto Alegre (RS), Brazil.
Laboratório de Helmintologia, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, UFRGS. Av. Bento Gonçalves, 9090, 91.501-970 Porto Alegre (RS), Brazil.
Vet Parasitol. 2016 Aug 15;226:162-6. doi: 10.1016/j.vetpar.2016.06.039. Epub 2016 Jul 1.
This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe. Other dyes currently used for staining biologic materials use organic solvents, which may be combined with acids or bases in their formulation, preventing their use with the tape method.
本研究提出了一种可视化马蛲虫卵的新方法，该方法通过将透明胶带粘贴于马的肛周区域来收集虫卵。所得结果表明，即使存在粪便和其他物质，该方案也能快速、简便、清晰且选择性地可视化马蛲虫卵。该方法的另一个优点是可与溶于水的荧光染料一起使用，这种染料能对生物材料进行染色而不溶解或改变胶带，且对环境安全。目前用于染色生物材料的其他染料使用有机溶剂，其配方中可能含有酸或碱，因此不能与胶带法一起使用。
