Qi Gaoxiang, Xiong Lian, Wang Bo, Lin Xiaoqing, Zhang Hairong, Li Hailong, Huang Chao, Chen Xuefang, Wang Can, Chen Xinde
Key Laboratory of Renewable Energy, Chinese Academy of Sciences, No.2 Nengyuan Road, Tianhe District, Guangzhou, 510640, People's Republic of China.
Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People's Republic of China.
Appl Biochem Biotechnol. 2017 Jan;181(1):177-191. doi: 10.1007/s12010-016-2206-5. Epub 2016 Aug 11.
Lithium chloride (LiCl)/N,N-dimethylacetamide (DMAc) solvent system was used to dissolve native and pretreated wheat straw materials in order to promote the enzymatic hydrolysis process. The dissolution ratio of wheat straw in LiCl/DMAc solvent system increased when dilute sulfuric acid or ethanol-sulfuric acid mixture pretreatment was conducted before dissolution. The materials regenerated from LiCl/DMAc solution exhibited obvious changes in structure and morphology, as revealed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. The cellulose and xylan digestibilities of regenerated materials were improved obviously. The cellulose digestibilities of materials regenerated from native and pretreated wheat straw by dilute sulfuric acid and ethanol-sulfuric acid mixture were 61.8, 81.2, and 84.4 %, respectively, with 25 FPU of cellulase and 187 IU of xylanase after 96 h. These values were significantly higher than the digestibilities of 0, 33.2, and 57.5 % obtained from corresponding materials before dissolution. Dissolution treatment of wheat straw by LiCl/DMAc solvent system provides an alternative method for efficient enzymatic hydrolysis.
采用氯化锂（LiCl）/N，N-二甲基乙酰胺（DMAc）溶剂体系溶解天然和预处理的小麦秸秆材料，以促进酶解过程。在溶解前进行稀硫酸或乙醇-硫酸混合预处理时，小麦秸秆在LiCl/DMAc溶剂体系中的溶解率增加。扫描电子显微镜（SEM）和X射线衍射（XRD）分析表明，从LiCl/DMAc溶液中再生的材料在结构和形态上呈现出明显变化。再生材料的纤维素和木聚糖消化率明显提高。在96小时后，使用25 FPU纤维素酶和187 IU木聚糖酶，天然小麦秸秆以及经稀硫酸和乙醇-硫酸混合预处理后再生材料的纤维素消化率分别为61.8%、81.2%和84.4%。这些值显著高于相应材料溶解前获得的0%、33.2%和57.5%的消化率。LiCl/DMAc溶剂体系对小麦秸秆进行溶解处理为高效酶解提供了一种替代方法。
