在一种非光合硅藻——白色菱形藻中鉴定出游离和共轭甾醇为24-亚甲基胆固醇。

Identification of the free and conjugated sterol in a non-photosynthetic diatom,Nitzschia alba, as 24-methylene cholesterol.

作者信息

Kates M, Tremblay P, Anderson R, Volcani B E

机构信息

Department of Biochemistry, University of Ottawa, K1N 6N5, Ottawa, Canada.

Scripps Institution of Oceanography, University of California, San Diego, 92093, La Jolla, California.

出版信息

Lipids. 1978 Jan;13(1):34-41. doi: 10.1007/BF02533364.

DOI:10.1007/BF02533364

PMID:27519997

Abstract

Previous studies on the sterol fraction of the nonphotosynthetic marine diatom,Nitszchia alba, indicated the major sterol to be either brassicasterol (24R-methylcholesta-5,22-dien-3β-ol) or 22-dehydrocampesterol (24S-methylcholesta-5,22-dien-3β-ol) on the basis only of gas chromatographymass spectral analysis. The present studies using nuclear magnetic resonance, infrared, and gas chromatography-mass spectrometry on the free and bound sterol fractions isolated by preparative thin layer chromatography showed the presence in both fractions of a single sterol, with spectral and chromatographic properties identical with those reported for 24-methylenecholesterol (ergosta-5,24(28)-dien-3β-ol). This sterol may be the precursor of 24-methyl sterols found in diatoms. The bound sterol fraction was found to consist of a single compound identified as 24-methylenecholesterol sulfate. No sterol esters or sterol glycosides were detected.

摘要

先前对非光合海洋硅藻——白色菱形藻（Nitszchia alba）甾醇组分的研究表明，仅基于气相色谱 - 质谱分析，主要甾醇为油菜甾醇（24R - 甲基胆甾 - 5,22 - 二烯 - 3β - 醇）或22 - 脱氢胆固醇（24S - 甲基胆甾 - 5,22 - 二烯 - 3β - 醇）。目前通过制备型薄层色谱法分离游离和结合甾醇组分，并使用核磁共振、红外光谱以及气相色谱 - 质谱联用技术进行的研究表明，两个组分中均存在单一甾醇，其光谱和色谱性质与报道的24 - 亚甲基胆固醇（麦角甾 - 5,24(28) - 二烯 - 3β - 醇）相同。这种甾醇可能是硅藻中发现的24 - 甲基甾醇的前体。发现结合甾醇组分由一种鉴定为24 - 亚甲基胆固醇硫酸盐的单一化合物组成。未检测到甾醇酯或甾醇糖苷。