Véron B, Billard C, Dauguet J C, Hartmann M A
Laboratoire de Biologie et Biotechnologies Marines, Université de Caen Basse-Normandie, France.
Lipids. 1996 Sep;31(9):989-94. doi: 10.1007/BF02522694.
In a detailed sterol analysis of the marine diatom Phaeodactylum tricornutum, free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13 degrees C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, except steryl esters, (24S)-24-methylcholesta-5,22E-dien-3 beta-ol (epibrassicasterol) was the major (80 to 99%) sterol component. Eight other sterols were identified. Growth under different light spectral quality (red, blue, yellow, and green) at 13 and 23 degrees C was examined. At 23 degrees C, a dramatic decrease in total sterol content was observed, especially under blue light. The distribution of sterols between free and conjugated forms as well as sterol profile inside each class was found to be strongly dependent on the light spectral quality at both temperatures.
在对海洋硅藻三角褐指藻进行的详细甾醇分析中，发现了游离甾醇以及酯化和糖基化共轭物。当该藻类在标准条件下（即在13摄氏度白光下）生长时，总甾醇的64%为甾醇糖苷。在所有甾醇类别中，除了甾醇酯外，（24S）-24-甲基胆甾-5,22E-二烯-3β-醇（表油菜甾醇）是主要的（80%至99%）甾醇成分。还鉴定出了其他八种甾醇。研究了在13摄氏度和23摄氏度下不同光谱质量（红色、蓝色、黄色和绿色）的光照条件下的生长情况。在23摄氏度时，观察到总甾醇含量急剧下降，尤其是在蓝光下。发现在这两个温度下，游离和共轭形式之间的甾醇分布以及每类甾醇的组成都强烈依赖于光谱质量。
