Coelho Aline Guadalupe, de Jesus Dosil Pereira
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, Brazil.
Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, Brazil.
Electrophoresis. 2016 Nov;37(22):2986-2991. doi: 10.1002/elps.201600263. Epub 2016 Sep 22.
In this work, a novel and simple analytical method using capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C D) is proposed for the determination of the polyols erythritol, maltitol, xylitol, and sorbitol in sugar-free chocolate. CE separation of the polyols was achieved in less than 6 min, and it was mediated by the interaction between the polyols and the borate ions in the background electrolyte, forming negatively charged borate esters. The extraction of the polyols from the samples was simply obtained using ultra-pure water and ultrasonic energy. Linearity was assessed by calibration curves that showed R varying from 0.9920 to 0.9976. The LOQs were 12.4, 15.9, 9.0, and 9.0 μg/g for erythritol, maltitol, xylitol, and sorbitol, respectively. The accuracy of the method was evaluated by recovery tests, and the obtained recoveries varied from 70 to 116% with standard deviations ranging from 0.2 to 19%. The CE-C D method was successfully applied for the determination of the studied polyols in commercial samples of sugar-free chocolate.
在本研究中，提出了一种新颖且简单的分析方法，即采用毛细管电泳（CE）结合电容耦合非接触电导检测（C D）来测定无糖巧克力中的多元醇赤藓糖醇、麦芽糖醇、木糖醇和山梨醇。多元醇的CE分离在不到6分钟内即可完成，其分离是通过多元醇与背景电解质中的硼酸根离子之间的相互作用介导的，形成带负电荷的硼酸酯。使用超纯水和超声能量即可简单地从样品中提取多元醇。通过校准曲线评估线性，曲线显示R值在0.9920至0.9976之间变化。赤藓糖醇、麦芽糖醇、木糖醇和山梨醇的定量限分别为12.4、15.9、9.0和9.0 μg/g。通过回收率测试评估该方法的准确性，获得的回收率在70%至116%之间，标准偏差在0.2%至19%之间。CE-C D方法成功应用于无糖巧克力商业样品中所研究多元醇的测定。
