Shaw R, Elliott W H
Edward A. Doisy Department of Biochemistry, Saint Louis University School of Medicine, 63104, St. Louis, MO.
Lipids. 1980 Oct;15(10):805-10. doi: 10.1007/BF02534369.
Allo bile acids and their taurine- and glycine-conjugates were synthesized by modification of existing procedures. Their chromatographic and spectral properties were investigated and compared with the 5β-analogs. Some of the naturally occurring 5α- and 5β-conjugates were separable by high performance liquid chromatography via straight-phase (Corasil II column) or reverse-phase systems (μBondapak/C18 column), though unsuccessful with thin layer or gas liquid chromatography. Differences between the C-5 epimers in the infrared and proton magnetic resonance spectra could be attributed to the configurations at the A/B ring junction and the configuration of the 3-hydroxyl group. The calcium salts of the allo compounds were generally less soluble in water than the 5β-isomers.
通过改进现有方法合成了别胆酸及其与牛磺酸和甘氨酸的共轭物。研究了它们的色谱和光谱性质，并与5β-类似物进行了比较。一些天然存在的5α-和5β-共轭物可通过正相（Corasil II柱）或反相系统（μBondapak/C18柱）的高效液相色谱法分离，不过在薄层色谱法或气液色谱法中未成功。C-5差向异构体在红外光谱和质子磁共振光谱中的差异可归因于A/B环连接处的构型和3-羟基的构型。别胆化合物的钙盐通常比5β-异构体在水中的溶解度小。
