Nakamura T, Maeda H, Takahashi Y, Hama Y
Laboratory of Fisheries Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812, Fukuoka, Japan.
Lipids. 1990 Sep;25(9):578-80. doi: 10.1007/BF02537169.
This report deals with the fluorometric determination of fatty alcohols generated by the reduction of the ester linkage of lipids with NaBH4, and with the limitations of the reduction method for assaying oxidized lipids. Optimum conditions for the fluorometric analysis of primary and secondary alcohols using 1-anthroyl nitrile were obtained. After reduction with NaBH4 in MeOH or in MeOH/benzene (8∶2, v/v), the formation of 1-hexadecanol from a variety of palmitic acid esters was measured fluorometrically by reverse-phase high-performance liquid chromatography (HPLC): From glycerides and methyl palmitate, 1-3% (w/w) 1-hexadecanol was produced and a trace was produced from cholesteryl palmitate (10 min, 21°C). 1-Hexadecanol was never generated from palmitic acid. Although considerable improvement occurred with the choice of the solvent for the NaBH4 reduction, the generation of primary alcohols from ester lipids usually seems inevitable.
本报告涉及用荧光法测定用硼氢化钠还原脂质酯键所生成的脂肪醇，以及该还原法在分析氧化脂质时的局限性。获得了使用1-蒽腈对伯醇和仲醇进行荧光分析的最佳条件。在甲醇或甲醇/苯（8∶2，v/v）中用硼氢化钠还原后，通过反相高效液相色谱（HPLC）荧光法测定了多种棕榈酸酯生成1-十六醇的情况：从甘油酯和棕榈酸甲酯中产生了1-3%（w/w）的1-十六醇，而从棕榈酸胆固醇酯中产生了微量的1-十六醇（10分钟，21°C）。棕榈酸从未生成1-十六醇。尽管在选择硼氢化钠还原的溶剂方面有了相当大的改进，但从酯类脂质中生成伯醇似乎通常是不可避免的。
