Saitoh Yasukazu, Ohta Hiroaki, Hyodo Sayuri
Laboratory of Bioscience & Biotechnology for Cell Function Control, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
Laboratory of Bioscience & Biotechnology for Cell Function Control, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
J Photochem Photobiol B. 2016 Oct;163:22-9. doi: 10.1016/j.jphotobiol.2016.08.001. Epub 2016 Aug 2.
To identify compounds that suppress UV irradiation-induced oxidative stress in the skin, various types of antioxidants have been studied. Polyvinylpyrrolidone-entrapped fullerene (C60/PVP) is known as a powerful antioxidant that exerts a cytoprotective effect against UV irradiation-induced cell injury in human skin cells and skin models. However, the effects of the alternate attractive C60/PVP feature, persistent antioxidant ability, on cytoprotection have rarely been ascertained. In this study we therefore investigated the efficacies of C60/PVP using an intermittently repeated UVA irradiation model wherein human keratinocytes were repeatedly exposed to UVA five times every 1h and compared the cytoprotective effects with those provided by ascorbic acid-2-O-phosphate-disodium salt (APS) and α-tocopherol (α-Toc). Our results demonstrated that C60/PVP yielded prominent cytoprotective effects against intermittently repeated UVA irradiation-induced injuries in a dose-dependent manner and suppressed intracellular superoxide anion radical (O2(-)) generation both during and after the repeated UVA irradiation. Additionally, C60/PVP also repressed the intermittent UVA irradiation-induced apoptosis via suppression of chromatin condensation and caspase-3/7 activation. Furthermore, the observed cytoprotective effects were superior to the effects of the typical antioxidants APS and α-Toc. These data suggest that C60/PVP might function as a potent cosmetic antioxidant against the effects of repeated and prolonged UVA irradiation through its persistent antioxidative property.
为了鉴定能够抑制紫外线照射引起的皮肤氧化应激的化合物，人们对各种类型的抗氧化剂进行了研究。聚乙烯吡咯烷酮包裹的富勒烯（C60/PVP）是一种强大的抗氧化剂，对紫外线照射引起的人类皮肤细胞和皮肤模型的细胞损伤具有细胞保护作用。然而，C60/PVP另一个吸引人的特性，即持久的抗氧化能力，对细胞保护的影响却鲜有研究。因此，在本研究中，我们使用间歇性重复UVA照射模型来研究C60/PVP的功效，该模型中人类角质形成细胞每隔1小时重复暴露于UVA 5次，并将其细胞保护作用与抗坏血酸-2-O-磷酸二钠盐（APS）和α-生育酚（α-Toc）的细胞保护作用进行比较。我们的结果表明，C60/PVP对间歇性重复UVA照射引起的损伤具有显著的剂量依赖性细胞保护作用，并在重复UVA照射期间和之后均抑制细胞内超氧阴离子自由基（O2(-)）的产生。此外，C60/PVP还通过抑制染色质浓缩和caspase-3/7激活来抑制间歇性UVA照射诱导的细胞凋亡。此外，观察到的细胞保护作用优于典型抗氧化剂APS和α-Toc的作用。这些数据表明，C60/PVP可能通过其持久的抗氧化特性，作为一种有效的化妆品抗氧化剂来对抗重复和长时间UVA照射的影响。
