Zhou Fang, Jia Xiaoling, Yang Yang, Yang Qingmao, Gao Chao, Zhao Yunhui, Fan Yubo, Yuan Xiaoyan
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
Mater Sci Eng C Mater Biol Appl. 2016 Nov 1;68:623-631. doi: 10.1016/j.msec.2016.06.036. Epub 2016 Jun 11.
The efficiency of biomaterials used in small vascular repair depends greatly on their ability to interact with vascular endothelial cells (VECs). Rapid endothelialization of the vascular grafts is a promising way to prevent thrombosis and intimal hyperplasia. In this work, modification of electrospun membranes of poly(ethylene glycol)-b-poly(l-lactide-co-ε-caprolactone) (PELCL) by three different peptides for regulation of VECs were studied in order to obtain ideal bioactive biomaterials as small diameter vascular grafts. QK (a mimetic peptide to vascular endothelial growth factor), Arg-Glu-Asp-Val (REDV, a specific adhesive peptide to VECs) and Val-Ala-Pro-Gly (VAPG, a specific adhesive peptide to vascular smooth muscle cells) were investigated. Surface properties of the modified membranes and the response of VECs were verified. It was found that protein adsorption and platelet adhesion were effectively suppressed with the introduction of QK, REDV or VAPG peptides on the PELCL electrospun membranes. Both QK- and REDV-modified electrospun membranes could accelerate the proliferation of VECs in the first 9days, and the QK-modified electrospun membrane promoted cell proliferation more significantly than the REDV-modified one. The REDV-modified PELCL membrane was the most favorable for VECs adhesion than QK- and VAPG-modified membranes. It was suggested that QK- or REDV-modified PELCL electrospun membranes may have great potential applications in cardiovascular biomaterials for rapid endothelialization in situ.
用于小血管修复的生物材料的效率在很大程度上取决于它们与血管内皮细胞（VECs）相互作用的能力。血管移植物的快速内皮化是预防血栓形成和内膜增生的一种有前景的方法。在这项工作中，研究了用三种不同的肽对聚（乙二醇）-b-聚（L-丙交酯-共-ε-己内酯）（PELCL）电纺膜进行修饰以调节VECs，以便获得理想的生物活性生物材料作为小直径血管移植物。研究了QK（血管内皮生长因子的模拟肽）、精氨酸-谷氨酸-天冬氨酸-缬氨酸（REDV，VECs的特异性黏附肽）和缬氨酸-丙氨酸-脯氨酸-甘氨酸（VAPG，血管平滑肌细胞的特异性黏附肽）。验证了修饰膜的表面性质和VECs的反应。结果发现，在PELCL电纺膜上引入QK、REDV或VAPG肽可有效抑制蛋白质吸附和血小板黏附。QK和REDV修饰的电纺膜在前9天均可加速VECs的增殖，且QK修饰的电纺膜比REDV修饰的电纺膜更显著地促进细胞增殖。REDV修饰的PELCL膜比QK和VAPG修饰的膜对VECs黏附最有利。提示QK或REDV修饰的PELCL电纺膜在心血管生物材料原位快速内皮化方面可能具有巨大的潜在应用价值。
