Erdman V V, Nasibullin T R, Tuktarova I A, Somova R Sh, Mustafina O E
Genetika. 2016 Apr;52(4):474-81.
Seeking human longevity association with gene polymorphisms in transcription factors in the Tatar ethnic group, we conducted an analysis for age-related genotype, frequencies in polymorphic sites of FOXO1A (rs4943794, 72327C>G) and FOXO3A (rs3800231, 35-2764A>G) genes. Genotyping was conducted by using the PCR-RFLP approach. According to the results of logistic regression analysis, during maturity and old age periods, a decrease in the number of FOXO1A*G/G (OR = 0.984, P = 0.004) genotype carriers occurs and an increase in the number of FOXO1AC/G (OR = 1.035, P = 0.014) and FOXO1AC/C (OR = 1.024, P = 0.033) genotype carriers occurs in the sample of subjects before gender adjustments. In the sample of long-livers, the number of FOXO1AC/C (OR = 0.772, P = 0.028) genotype carriers decreased among women, while the number of FOXO3AG/*G (OR = 1.008, P = 0.0001) genotype carriers increased among both men and women. Therefore, the FOXO1A gene polymorphic site rs4943794 is associated with an acquisition of old and senescent age in a sample before gender adjustments and with women's longevity. FOXO3A gene polymorphic site rs3800231 is associated with longevity in both women and men.
为了探寻鞑靼族转录因子基因多态性与人类长寿之间的关联，我们对FOXO1A（rs4943794，72327C>G）和FOXO3A（rs3800231，35-2764A>G）基因多态性位点的年龄相关基因型及频率进行了分析。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。根据逻辑回归分析结果，在成年和老年期，未进行性别调整的研究对象样本中，FOXO1A*G/G（OR = 0.984，P = 0.004）基因型携带者数量减少，而FOXO1AC/G（OR = 1.035，P = 0.014）和FOXO1AC/C（OR = 1.024，P = 0.033）基因型携带者数量增加。在长寿者样本中，女性中FOXO1AC/C（OR = 0.772，P = 0.028）基因型携带者数量减少，而男性和女性中FOXO3AG/*G（OR = 1.008，P = 0.0001）基因型携带者数量均增加。因此，在未进行性别调整的样本中，FOXO1A基因多态性位点rs4943794与老年和衰老的获得以及女性长寿相关。FOXO3A基因多态性位点rs3800231与男性和女性的长寿均相关。
