Kuskov A N, Kulikov P P, Shtilman M I, Rakitskii V N, Tsatsakis A M
D.I. Mendeleyev University of Chemical Technology of Russia, Moscow 125047, Russian Federation; Moscow State University of Mechanical Engineering (MAMI), Moscow 107023, Russian Federation.
D.I. Mendeleyev University of Chemical Technology of Russia, Moscow 125047, Russian Federation.
Food Chem Toxicol. 2016 Oct;96:273-9. doi: 10.1016/j.fct.2016.08.017. Epub 2016 Aug 15.
The aim of the present study was to evaluate the cytotoxicity against MCF-7 cells and acute intraperitoneal toxicity of amphiphilic poly-N-vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In acute toxicity studies, LD50 and other toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly-N-vinylpyrrolidone nanoparticles possessed no toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems.
本研究的目的是评估两亲性聚N-乙烯基吡咯烷酮纳米颗粒对MCF-7细胞的细胞毒性和急性腹腔毒性，以确认其用于创建新型药物递送系统的可能性。通过MTT法研究了聚合物纳米颗粒对人癌细胞系MCF-7细胞的细胞摄取作用。MTT分析表明，所测试的两亲性聚合物基本无毒。在急性毒性研究中，评估了LD50和其他毒性指标，在实验的14天高浓度处理下，甚至未观察到死亡或与治疗相关的并发症。为了进行组织学分析，对动物的器官进行称重和检查。研究期间没有动物死亡，在体重、饲料消耗、器官重量或组织学数据方面也未观察到显著变化。获得的结果表明，两亲性聚N-乙烯基吡咯烷酮纳米颗粒经腹腔给药后对细胞和动物均无毒性。因此，两亲性PVP纳米颗粒作为新型高效药物递送系统的载体具有很高的潜力。
