Osama Amany, Sabry Dina, Hassany Sahar M, Abdelmoneim Soha Saoud, Sabry Abeer
Biochemistry Department, Assiut University, Assiut, Egypt.
Medical Biochemistry and Molecular Biology Department, Cairo University, Cairo, Egypt.
Cancer Biomark. 2016 Jun 24;17(2):155-63. doi: 10.3233/CBM-160626.
The study aimed to investigate the quantitative expression of NANOG, p38 α , NCF2, ELF and TGF-β genes in patients with colorectal adenocarcinoma, adenoma and normal colonic tissue and their correlation with SIRT-1 protein level expression.
This study enrolled one hundred sixty seven patients; group A: 87 patients with colonoscopic findings of no adenoma or adenocarcinoma and group B: 80 patients with colorectal mass. Consecutive colonoscopic examinations were conducted, and tissue samples were taken from the colonic lesions/masses. Total RNA was isolated and mRNA expression level of NANOG, mitogen activated p38α , Neutrophil Cytosol Factor 2 (NCF2), Embryonic Liver Fodrin (ELF) and Transforming Growth Factor Beta (TGF-β) genes were quantified by qRT-PCR. Sirt-1 protein expression level was assessed by quantitative western blot.
There were significantly high level of mRNA transcripts expression of the genes studied in patients with adenocarcinoma and adenoma compared with normal tissue (P value < 0.01), NANOG, NCF2, ELF and TGF-β at a cut of > 0.314, > 0.392, 0.349 and 0.333 respectively showed sensitivity (96.5%, 98.8%, 95.3%, 98.8%) and specificity of (95.3%, 92.6%, 89.5%, 93.8%) respectively in diagnosing colonic adenocarcinoma. Sirt-1 protein level was significantly highly expressed in colorectal adenocarcinoma compared to normal and adenoma colonic tissue and positively correlated with NANOG.
Over expression of NANOG, p38α , NCF2, ELF and TGF-β genes in both cases of adenocarcinoma and adenoma could have a diagnostic value. SIRT-1 and NANOG are high correlated biological markers for diagnosis and prognosis follow up in patients with adenocarcinoma.
本研究旨在调查结直肠癌、腺瘤及正常结肠组织患者中NANOG、p38α、NCF2、ELF和TGF-β基因的定量表达及其与SIRT-1蛋白水平表达的相关性。
本研究纳入了167例患者；A组：87例经结肠镜检查未发现腺瘤或腺癌的患者，B组：80例患有结直肠肿物的患者。进行连续的结肠镜检查，并从结肠病变/肿物处采集组织样本。提取总RNA，通过qRT-PCR定量NANOG、丝裂原活化p38α、中性粒细胞胞浆因子2（NCF2）、胚胎肝肌动蛋白（ELF）和转化生长因子β（TGF-β）基因的mRNA表达水平。通过定量蛋白质免疫印迹法评估Sirt-1蛋白表达水平。
与正常组织相比，腺癌和腺瘤患者中所研究基因的mRNA转录本表达水平显著升高（P值<0.01），NANOG、NCF2、ELF和TGF-β在截断值分别>0.314、>0.392、0.349和0.333时，诊断结肠腺癌的敏感性分别为（96.5%、98.8%、95.3%、98.8%），特异性分别为（95.3%、92.6%、89.5%、93.8%）。与正常和腺瘤性结肠组织相比，Sirt-1蛋白水平在结直肠癌中显著高表达，且与NANOG呈正相关。
腺癌和腺瘤病例中NANOG、p38α、NCF2、ELF和TGF-β基因的过表达可能具有诊断价值。SIRT-1和NANOG是腺癌患者诊断和预后随访的高度相关生物学标志物。
