Van Veldhoven P P, Foglesong R J, Bell R M
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
J Lipid Res. 1989 Apr;30(4):611-6.
A procedure is described to prepare sphingosine-1-phosphate by treatment of sphingosylphosphocholine with phospholipase D, isolated from Streptomyces chromofuscus. The phosphorylated long chain bases were purified by selective precipitation and differential extraction. Milligram quantities can be obtained in a yield of about 70%. Application of the procedure to dihydrosphingosylphosphocholine results in the synthesis of dihydrosphingosine-1-phosphate.
描述了一种通过用从暗褐链霉菌中分离的磷脂酶D处理鞘氨醇磷酸胆碱来制备鞘氨醇-1-磷酸的方法。通过选择性沉淀和差异萃取对磷酸化的长链碱基进行纯化。可以获得毫克量，产率约为70%。将该方法应用于二氢鞘氨醇磷酸胆碱可合成二氢鞘氨醇-1-磷酸。
