Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Asian Pac J Allergy Immunol. 2016 Dec;34(4):314-323. doi: 10.12932/AP0734.
MF59, which is an adjuvant belonging to C₃₀ member of the terpene family, is a T helper type-2 (Th2)-biased immune enhancer. Our previous studies showed that pyriproxyfen, a member of the terpene family with fewer carbon atoms (C₂₀) than MF59, enhanced active T helper type-1 (Th1)-biased immune responses.
This study was performed to investigate the enhancement of antigen-specific immune responses by myrcene, a member of the terpene family with fewer carbon atoms (C₁₀) than pyriproxyfen.
Ovalbumin (OVA) was used as an antigen to determine the effects of myrcene on the immune response. The IgG subtypes and cytokines induced by immunization of OVA with or without myrcene were monitored. Thereafter, we determined the effects of myrcene in the immune response against Ag85B, which is a dominant protective antigen for tuberculosis.
The results showed that 0.8 mg/dose of myrcene enhanced antigen-specific total IgG immune response to OVA. Direct mixing of the antigen with myrcene was required for the enhancement of antibody production. Myrcene increased OVA-specific IgG2a titer, suggesting induction of Th1-immune response. The level of Th1 cytokines, IFN-γ was increased at 8 weeks after immunization, although IL-13 was also increased at the same time point. However, finally myrcene was found to increase Ag85B-specific total IgG titers at 5 weeks and specific IgG2a titer was increased at both 5 and 8 weeks. The results suggested that myrcene could enhance Th1 immune response.
Myrcene enhanced specific immune responses against OVA and Ag85B. This study suggested the tendency of the enhancement of Th1 immune response by myrcene.
MF59 是一种属于三萜烯家族 C₃₀成员的佐剂，是一种 Th2 偏向的免疫增强剂。我们之前的研究表明，比 MF59 少 20 个碳原子（C₂₀）的拟除虫菊酯能增强主动 Th1 偏向的免疫反应。
本研究旨在探讨比拟除虫菊酯少 10 个碳原子（C₁₀）的苎烯对特定抗原免疫反应的增强作用。
以卵清蛋白（OVA）为抗原，观察苎烯对免疫反应的影响。监测免疫接种 OVA 前后诱导的 IgG 亚型和细胞因子。然后，我们确定了苎烯在针对结核分枝杆菌主要保护性抗原 Ag85B 的免疫反应中的作用。
结果表明，0.8mg/d 剂量的苎烯增强了 OVA 特异性总 IgG 免疫反应。抗原与苎烯直接混合是产生抗体增强的必要条件。苎烯增加了 OVA 特异性 IgG2a 滴度，提示诱导了 Th1 免疫反应。Th1 细胞因子 IFN-γ的水平在免疫后 8 周增加，尽管同时也增加了 IL-13。然而，最终发现苎烯能在 5 周时增加 Ag85B 特异性总 IgG 滴度，在 5 周和 8 周时特异性 IgG2a 滴度增加。结果表明苎烯能增强针对 OVA 和 Ag85B 的特异性免疫反应。本研究提示苎烯可能增强 Th1 免疫反应。
苎烯增强了针对 OVA 和 Ag85B 的特异性免疫反应。本研究提示苎烯有增强 Th1 免疫反应的倾向。
