Twist1在小鼠植入前胚胎中的差异表达模式表明其在早期发育过程中具有多种作用。
Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development.
作者信息
Sozen Berna, Pehlivanoglu Suray, Demir Necdet
机构信息
Department of Histology and Embryology, School of Medicine, Akdeniz University Campus, 07070, Antalya, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, 42090, Konya, Turkey.
出版信息
J Assist Reprod Genet. 2016 Nov;33(11):1533-1540. doi: 10.1007/s10815-016-0794-1. Epub 2016 Aug 20.
PURPOSE
The purpose of the present study is to understand Twist-related protein 1 (Twist1) spatiotemporal expression patterns and functions during early embryo development.
METHODS
We performed whole-mount double immunofluorescence staining and reverse transcription (RT)-PCR analysis of the Twist1 protein and gene throughout the preimplantation development in mice.
RESULTS
We determined that after compaction, the expression of Twist1 becomes developmentally differentiated and targeted in the inner cells of embryos. In blastocysts at E4.5, uniform staining of the inner cell mass was apparent, and it had been gradually translocated to the nucleus of hatched embryonic cells at E4.75. Furthermore, the effect of potential regulators of Twist on its expression level during blastocyst development was also sought. Accordingly, Twist1 expression appeared to be upregulated in both mRNA and protein level following culture of embryos in the presence of high glucose.
CONCLUSIONS
Our study revealed the dynamic Twist localization within the early stage of embryo. The results are discussed in terms of potential roles of Twist1 in the processes of lineage segregation, hatching, and implantation in post-compaction embryos and in blastocysts.
目的
本研究旨在了解Twist相关蛋白1（Twist1）在早期胚胎发育过程中的时空表达模式及功能。
方法
我们对小鼠植入前发育全过程的Twist1蛋白和基因进行了全胚胎双免疫荧光染色及逆转录（RT）-PCR分析。
结果
我们确定在致密化后，Twist1的表达在胚胎内部细胞中呈现出发育分化和靶向性。在E4.5的囊胚中，内细胞团出现均匀染色，并且在E4.75时逐渐转移至孵化胚胎细胞的细胞核中。此外，还研究了Twist潜在调节因子对其在囊胚发育过程中表达水平的影响。相应地，在高糖环境下培养胚胎后，Twist1的mRNA和蛋白水平均出现上调。
结论
我们的研究揭示了胚胎早期阶段Twist的动态定位。就Twist1在致密化后胚胎和囊胚的谱系分离、孵化及着床过程中的潜在作用进行了讨论。
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