School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, South Korea.
FASEB J. 2019 Oct;33(10):11326-11337. doi: 10.1096/fj.201900370RR. Epub 2019 Jul 19.
In mammals, the early embryo travels down the oviduct to the uterus and prepares for implantation. The unique features of preimplantation development include compaction followed by blastocyst formation. This first cell lineage specification involves various proteins including cell polarity regulators, kinases, and transcription factors. In this study, a novel gene named predicted gene 11545 () expressed predominantly in mouse early embryos was identified and characterized at the transcript, protein, cellular, and functional levels. The Gm11545 protein localized to both cytoplasmic and membrane regions of preimplantation embryos. Remarkably, knockdown of led to arrest of mouse embryos at the morula stage and consequent impairment of blastocyst formation. Expression patterns of the key transcription factors critical for early lineage specification, octamer-binding transcription factor 4 and caudal type homeobox 2, were affected by depletion. Based on the collective findings, we propose that the novel protein identified in this study, Gm11545, is implicated in cell proliferation and cell lineage specification critical for blastocyst formation.-Kim, J., Kim, J., Jeong, J., Hong, S. H., Kim, D., Choi, S., Choi, I., Oh, J. S., Cho, C. Identification of a novel embryo-prevalent gene, , involved in preimplantation embryogenesis in mice.
在哺乳动物中，早期胚胎沿着输卵管移动到子宫，并为着床做准备。着床前发育的独特特征包括致密化，随后形成囊胚。这第一次细胞谱系特化涉及各种蛋白质，包括细胞极性调节剂、激酶和转录因子。在这项研究中，鉴定并表征了一种新型基因，命名为预测基因 11545()，在转录本、蛋白质、细胞和功能水平上，它主要在小鼠早期胚胎中表达。Gm11545 蛋白定位于着床前胚胎的细胞质和膜区域。值得注意的是，下调导致小鼠胚胎停滞在桑葚胚阶段，并随后损害囊胚形成。关键转录因子的表达模式对于早期谱系特化至关重要，八聚体结合转录因子 4 和尾型同源盒 2，受到 耗竭的影响。基于这些综合发现，我们提出，在这项研究中鉴定的新型蛋白 Gm11545 参与了囊胚形成中关键的细胞增殖和细胞谱系特化。-Kim, J., Kim, J., Jeong, J., Hong, S. H., Kim, D., Choi, S., Choi, I., Oh, J. S., Cho, C.Identification of a novel embryo-prevalent gene,, involved in preimplantation embryogenesis in mice.
