Wiesak T
Department of Animal Physiology, University of Agriculture and Technology, Olsztyn, Poland.
J Reprod Fertil. 1989 May;86(1):247-54. doi: 10.1530/jrf.0.0860247.
Corpora lutea were obtained from pig ovaries on Day 18 of pregnancy or pseudopregnancy. Pseudopregnancy was induced by the administration of oestradiol benzoate on Days 11-15 of the oestrous cycle or by the administration of hCG on Day 12. The luteal cells were prepared for morphometric analysis and investigation of steroid production in vitro by dispersion with 0.25% trypsin. A blood sample from each sow was collected at slaughter for measurement of progesterone, oestradiol-17 beta and testosterone. The concentrations of these steroids were also estimated in luteal tissue and in the medium after incubation. Progesterone concentration was significantly higher (P less than 0.01) in luteal tissue and in plasma of pregnant than of pseudopregnant sows. Testosterone content of luteal tissue from all sows was 20-fold higher than oestradiol, although plasma concentrations of these hormones were not different. The luteal cells from hCG-treated sows produced more progesterone (P less than 0.01) in vitro than did those from the other groups. The luteal cells from oestradiol-treated sows generally released smaller amounts of steroids during incubation. Treatment with hCG increased the proportion of large luteal cells and decreased the proportion of small luteal cells. These results demonstrate that hCG or oestradiol benzoate injections altered the steroidogenic activity of luteal cells and that treatment with hCG was also associated with changes in the diameter of the luteal cells and thus in the ratio of small to large luteal cells.
在妊娠或假妊娠第18天从猪卵巢获取黄体。假妊娠通过在发情周期的第11 - 15天注射苯甲酸雌二醇或在第12天注射人绒毛膜促性腺激素（hCG）诱导产生。用0.25%胰蛋白酶分散黄体细胞，用于形态计量分析和体外类固醇生成研究。在屠宰时从每头母猪采集血样，测定孕酮、雌二醇 - 17β和睾酮。这些类固醇的浓度也在黄体组织和孵育后的培养基中进行了估算。妊娠母猪黄体组织和血浆中的孕酮浓度显著高于假妊娠母猪（P < 0.01）。所有母猪黄体组织中的睾酮含量比雌二醇高20倍，尽管这些激素的血浆浓度没有差异。与其他组相比，经hCG处理的母猪的黄体细胞在体外产生更多的孕酮（P < 0.01）。经雌二醇处理的母猪的黄体细胞在孵育期间通常释放较少的类固醇。hCG处理增加了大黄体细胞的比例，降低了小黄体细胞的比例。这些结果表明，注射hCG或苯甲酸雌二醇改变了黄体细胞的类固醇生成活性，并且hCG处理还与黄体细胞直径的变化相关，从而与小黄体细胞与大黄体细胞的比例变化相关。
